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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other

Anti-MCM7/PRL抗体[47DC141] (ab2360)

  • Datasheet
Reviews (7) Submit a question References (26)

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Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody [47DC141] (ab2360)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [47DC141] (ab2360)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody [47DC141] (ab2360)
  • Flow Cytometry - Anti-MCM7/PRL antibody [47DC141] (ab2360)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [47DC141] (ab2360)

Key features and details

  • Mouse monoclonal [47DC141] to MCM7/PRL
  • Suitable for: IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-MCM7/PRL抗体[47DC141]
    参阅全部 MCM7/PRL 一抗
  • 描述

    小鼠单克隆抗体[47DC141] to MCM7/PRL
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性

    与反应: Rat, Human
  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 克隆

    单克隆
  • 克隆编号

    47DC141
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    unknown
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human MCM7/PRL protein (ab114356)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2360于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (2)
1/50 - 1/100. This is when using an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Flow Cyt
1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
Use a concentration of 1 µg/ml.
说明
IHC-P
1/50 - 1/100. This is when using an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Flow Cyt
1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
  • 序列相似性

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession P33993 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4176 Human
    • Entrez Gene: 288532 Rat
    • Omim: 600592 Human
    • SwissProt: P33993 Human
    • Unigene: 438720 Human
    • 别名

      • CDABP0042 antibody
      • CDC 47 antibody
      • CDC47 antibody
      • CDC47 homolog antibody
      • Cdc47, S. cerevisiae, homolog of antibody
      • DNA replication licensing factor MCM7 antibody
      • Homolog of S. cerevisiae Cdc47 antibody
      • MCM 2 antibody
      • MCM 7 antibody
      • MCM2 antibody
      • MCM2, formerly antibody
      • Mcm7 antibody
      • MCM7 minichromosome maintenance deficient 7 antibody
      • MCM7_HUMAN antibody
      • Minichromosome Maintainence 7 antibody
      • Minichromosome maintainence, S. cerevisiae, homolog of antibody
      • Minichromosome maintenance complex component 7 antibody
      • Minichromosome maintenance deficient 7 antibody
      • Minichromosome maintenance protein 7 antibody
      • P1.1 MCM3 antibody
      • P1.1-MCM3 antibody
      • P1CDC47 antibody
      • P85MCM antibody
      • PNAS 146 antibody
      • PNAS146 antibody
      • PPP1R104 antibody
      • Protein phosphatase 1, regulatory subunit 104 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody [47DC141] (ab2360)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody [47DC141] (ab2360)

      This image shows immunostaining of MCM7/PRL in rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody MCM7/PRL (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC.  Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.

      The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.

       
       
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [47DC141] (ab2360)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [47DC141] (ab2360)Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview

      ab2360 staining MCM7/PRL in human bladder cancer tissue sections by Immunohistochemistry (formalin fixed sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 5% BSA for 1 hour at room temperature followed by incubation with the primary antibody at a 1/1200 dilution for 1 hour. A HRP-conjugated goat anti-mouse polyclonal was used as secondary antibody un-diluted.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody [47DC141] (ab2360)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody [47DC141] (ab2360)

      ICC/IF image of MCM7/PRL (ab2360) stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - Anti-MCM7/PRL antibody [47DC141] (ab2360)
      Flow Cytometry - Anti-MCM7/PRL antibody [47DC141] (ab2360)

      Overlay histogram showing HeLA cells stained with MCM7/PRL (ab2360) (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2360, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [47DC141] (ab2360)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [47DC141] (ab2360)

      ab2360 staining MCM7/PRL in formalin-fixed, paraffin-embedded Human breast carcinoma tissue tissue by Immunohistochemistry.

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (26)

    发表研究结果有使用 ab2360?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2360 被引用在 26 文献中.

    • Yang Y  et al. MCM7 silencing promotes cutaneous melanoma cell autophagy and apoptosis by inactivating the AKT1/mTOR signaling pathway. J Cell Biochem 121:1283-1294 (2020). PubMed: 31535400
    • Niu Y  et al. Huaier Suppresses the Hepatocellular Carcinoma Cell Cycle by Regulating Minichromosome Maintenance Proteins. Onco Targets Ther 13:12015-12025 (2020). PubMed: 33244243
    • Caldeira-Brant AL  et al. A subpopulation of human Adark spermatogonia behaves as the reserve stem cell. Reproduction 159:437-451 (2020). PubMed: 31990675
    • Kovary KM  et al. Expression variation and covariation impair analog and enable binary signaling control. Mol Syst Biol 14:e7997 (2018). PubMed: 29759982
    • Drissi R  et al. Destabilization of the MiniChromosome Maintenance (MCM) complex modulates the cellular response to DNA double strand breaks. Cell Cycle 17:2593-2609 (2018). PubMed: 30516086
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-MCM7/PRL antibody [47DC141]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    60 µg
    Treatment
    MMC 2H
    Specification
    293T
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 19 2018

    Western blot abreview for Anti-MCM7/PRL antibody [47DC141]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (3T3MEF)
    Gel Running Conditions
    Non-reduced Denaturing (7.5% acrylamide-Bis gel)
    Loading amount
    50000 cells
    Treatment
    by serum starvation for 48h , cells are synchronized at G0 phase and released with 5% FBS and collected at indicated time
    Specification
    3T3MEF
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 18 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCM7/PRL antibody [47DC141]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
    Permeabilization
    Yes - with wash - Buffer with Tween
    Specification
    Tonsil
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Rudolf Jung

    Verified customer

    提交于 Jun 10 2013

    Western blot abreview for Anti-MCM7/PRL antibody [47DC141]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SW780 bladder cancer)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    25 µg
    Specification
    SW780 bladder cancer
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Karin Birkenkamp-Demtroeder

    Verified customer

    提交于 May 06 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCM7/PRL antibody [47DC141]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Bladder Cancer / Transitional cell Carcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Bladder Cancer / Transitional cell Carcinoma
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Karin Birkenkamp-Demtroeder

    Verified customer

    提交于 Apr 29 2010

    Western blot abreview for Anti-MCM7/PRL antibody [47DC141]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T (LANE 1 AND 2))
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    25 µg
    Specification
    293T (LANE 1 AND 2)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 31 2008

    Western blot abreview for Anti-MCM7/PRL antibody [47DC141]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Tissue lysate - whole (Egg extracts)
    Loading amount
    12 µg
    Specification
    Egg extracts
    Blocking step
    Milk as blocking agent for 20 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 25 2006

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