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AB96849

Anti-MCM7/PRL抗体

Anti-MCM7/PRL antibody

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(2 Publications)

Rabbit Polyclonal MCM7/PRL antibody. Suitable for WB, IP, ChIP, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCM7 aa 1 to C-terminus.

查看别名

CDC47, MCM2, MCM7, DNA replication licensing factor MCM7, CDC47 homolog, P1.1-MCM3

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (AB96849)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (AB96849)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96849 at 1/200 dilution (1).Merged with DNA probe (2).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody (AB96849)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody (AB96849)

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using ab96849 at 1/100 dilution.

Immunoprecipitation - Anti-MCM7/PRL antibody (AB96849)
  • IP

Supplier Data

Immunoprecipitation - Anti-MCM7/PRL antibody (AB96849)

MCM7 was immunoprecipitated from 40 μg of HeLa whole cell lysate with ab96849 at 1/1000 dilution. EasyBlot anti-rabbit IgG, was used as secondary antibody.
Lane 1 : 40 μg HeLa whole cell extract (Input).
Lane 2 : Control with 4 μg of preimmune Rabbit IgG.
Lane 3 : Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal.

7.5 % SDS-PAGE

All lanes:

Immunoprecipitation - Anti-MCM7/PRL antibody (ab96849)

Predicted band size: 81 kDa

false

ChIP - Anti-MCM7/PRL antibody (AB96849)
  • ChIP

Supplier Data

ChIP - Anti-MCM7/PRL antibody (AB96849)

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

ICC/IF, ChIP, WB, IP, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human MCM7 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P33993

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替代产品

Primary Antibodies

AB199753

Alexa Fluor® 488 Anti-MCM7/PRL antibody [EP1974Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MCM7/PRL antibody [EP1974Y] (AB199753)

  • 0
  • 0
Primary Antibodies

AB134194

Anti-MCM7/PRL antibody [EPR1973Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MCM7/PRL antibody [EPR1973Y] (AB134194)

  • 0
  • 0
Primary Antibodies

AB242382

Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (AB242382)

  • 0
  • 0
Primary Antibodies

AB52489

Anti-MCM7/PRL antibody [EP1974Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [EP1974Y] (AB52489)

  • 0
  • 0
Primary Antibodies

AB199818

Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (AB199818)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB108935

Anti-MCM2 antibody [EPR4120]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)

  • 0
  • 0
Primary Antibodies

AB201683

Anti-MCM6 antibody [EPR17686]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (AB201683)

  • 0
  • 0
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB3733

Anti-MCM10 antibody

Western blot - Anti-MCM10 antibody (AB3733)

  • 3
  • 1
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MCM7 (Minichromosome Maintenance Complex Component 7) also known as PRL serves an important mechanical role in DNA replication. It is part of the MCM2-7 complex a helicase essential for the initiation and elongation phases of DNA replication. MCM7 has a molecular mass of approximately 81 kDa and is found expressed in the nucleus of proliferating cells. It is essential for unwinding the DNA helix preparing it for replication.
Biological function summary

MCM7 is involved in maintaining genomic stability and ensuring accurate DNA replication. As part of the larger MCM2-7 hexameric complex it collaborates with other members to form the core component of the pre-replicative complex (pre-RC). By loading onto replication origins MCM7 helps license the DNA for duplication. This function contributes to cell cycle progression particularly the S phase where DNA synthesis occurs.

Pathways

MCM7 plays an important role in DNA replication-related signaling pathways. It functions within the cell cycle and DNA replication pathways interacting closely with other essential proteins like CDC45 and GINS complex. These partnerships facilitate the transition from the G1 to the S phase triggering the replication machinery's activation and ensuring cell division processes continue smoothly.

MCM7 has been associated with various cancers including breast and prostate cancer. Aberrant expression or dysregulation of MCM7 contributes to uncontrolled cell proliferation linking it to tumorigenesis. Also MCM7 interacts with proteins such as cyclin D1 in certain oncogenic pathways emphasizing its role in the progression of cancerous conditions. Understanding these interactions offers insights into potential therapeutic targets for cancer treatment.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 25661590, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for S-phase checkpoint activation upon UV-induced damage.
See full target information MCM7
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed40738669

2025

Histone Lactylation Promotes Proliferation and Migration of TNBC Cells via Upregulating MCM7 Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Xiao,Chaoxian Wang,Sili Jin,Ziran Zhang,Xingxia Yang,Guangfa Xia

Cell reports 7:575-587 PubMed24726359

2014

The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability.

Applications

WB

Species

Unspecified reactive species

Benoit Miotto,Moredreck Chibi,Ping Xie,Stéphane Koundrioukoff,Hanlie Moolman-Smook,David Pugh,Michelle Debatisse,Fuchu He,Lingqiang Zhang,Pierre-Antoine Defossez
View all publications
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