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重组RabMAb

重组Anti-MCM6抗体[EPR17686] (ab201683)

Submit a review Submit a question References (17)
Western blot - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Western blot - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Western blot - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Flow Cytometry (Intracellular) - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Immunoprecipitation - Anti-MCM6 antibody [EPR17686] (ab201683)
  • Anti-MCM6 antibody [EPR17686] (ab201683)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17686] to MCM6
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

概述

  • 产品名称

    Anti-MCM6抗体[EPR17686]
    参阅全部 MCM6 一抗

  • 描述

    兔单克隆抗体[EPR17686] to MCM6

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: MCF7, HeLa, Jurkat, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human tonsil, heart and kidney lysates. IHC-P: Human tonsil, rat liver and mouse stomach tissues. ICC/IF: Jurkat and MCF7 cells. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab201683于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/130.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/40.
说明
Flow Cyt (Intra)
1/130.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/40.

靶标

  • 功能

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

  • 序列相似性

    Belongs to the MCM family.
    Contains 1 MCM domain.

  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • DNA replication licensing factor MCM6 antibody
    • MCG40308 antibody
    • Mcm6 antibody
    • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
    • MCM6 minichromosome maintenance deficient 6 antibody
    • MCM6_HUMAN antibody
    • Minichromosome maintenance complex component 6 antibody
    • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
    • Minichromosome maintenance deficient 6 homolog antibody
    • Minichromosome maintenance protein 6 antibody
    • Mis5 antibody
    • MIS5 homolog antibody
    • p105MCM antibody
    see all

图片

  • Western blot - Anti-MCM6 antibody [EPR17686] (ab201683)
    Western blot - Anti-MCM6 antibody [EPR17686] (ab201683)
    All lanes : Anti-MCM6 antibody [EPR17686] (ab201683) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 4 : Human tonsil lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MCM6 antibody [EPR17686] (ab201683)
    Western blot - Anti-MCM6 antibody [EPR17686] (ab201683)
    All lanes : Anti-MCM6 antibody [EPR17686] (ab201683) at 1/1000 dilution

    Lane 1 : Human heart lysate
    Lane 2 : Human kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MCM6 antibody [EPR17686] (ab201683)
    Western blot - Anti-MCM6 antibody [EPR17686] (ab201683)
    All lanes : Anti-MCM6 antibody [EPR17686] (ab201683) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (ab201683)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (ab201683)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MCM6 with ab201683 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (ab201683)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (ab201683)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MCM6 with ab201683 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on rat liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (ab201683)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [EPR17686] (ab201683)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MCM6 with ab201683 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on mouse stomach tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [EPR17686] (ab201683)
    Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [EPR17686] (ab201683)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MCM6 with ab201683 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab201683 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [EPR17686] (ab201683)
    Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [EPR17686] (ab201683)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling MCM6 with ab201683 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab201683 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry (Intracellular) - Anti-MCM6 antibody [EPR17686] (ab201683)
    Flow Cytometry (Intracellular) - Anti-MCM6 antibody [EPR17686] (ab201683)

    Intracellular flow cytometric analysis of HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling MCM6 with ab201683 at 1/130 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730;black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-MCM6 antibody [EPR17686] (ab201683)
    Immunoprecipitation - Anti-MCM6 antibody [EPR17686] (ab201683)

    MCM6 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201683 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab201683 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab201683 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201683 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-MCM6 antibody [EPR17686] (ab201683)
    Anti-MCM6 antibody [EPR17686] (ab201683)

文献 (17)

发表研究结果有使用 ab201683？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab201683 被引用在 17 文献中.

  • Huang J  et al. Minichromosome maintenance 6 protects against renal fibrogenesis by regulating DUSP6-mediated ERK/GSK-3β/Snail1 signaling. iScience 26:107940 (2023). PubMed: 37810227
  • Zhang W  et al. NFIB facilitates replication licensing by acting as a genome organizer. Nat Commun 14:5076 (2023). PubMed: 37604829
  • Wang Y  et al. LSD1 is required for euchromatic origin firing and replication timing. Signal Transduct Target Ther 7:102 (2022). PubMed: 35414135
  • Polasek-Sedlackova H  et al. Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes. Nat Commun 13:6090 (2022). PubMed: 36241664
  • Lei Y  et al. Identification of MCM family as potential therapeutic and prognostic targets for hepatocellular carcinoma based on bioinformatics and experiments. Life Sci 272:119227 (2021). PubMed: 33607151
View all Publications for this product

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