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重组

重组Anti-MCM6抗体[1/MCM6] - BSA and Azide free (ab280215)

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Western blot - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
  • Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1/MCM6] to MCM6 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-MCM6抗体[1/MCM6] - BSA and Azide free
    参阅全部 MCM6 一抗

  • 描述

    小鼠单克隆抗体[1/MCM6] to MCM6 - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, ICC/IF, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 阳性对照

    • WB: MCF7, HeLa, SW480, U-2 OS and A431, Human tonsil lysates. IHC-P: Human colon and tonsil tissues. ICC: A431, U-2 OS cells.

  • 常规说明

    ab280215 is the carrier-free version of ab280208.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab280215于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 93 kDa.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 93 kDa.

靶标

  • 功能

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

  • 序列相似性

    Belongs to the MCM family.
    Contains 1 MCM domain.

  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Information by UniProt

  • 数据库链接

    • 别名

      • DNA replication licensing factor MCM6 antibody
      • MCG40308 antibody
      • Mcm6 antibody
      • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
      • MCM6 minichromosome maintenance deficient 6 antibody
      • MCM6_HUMAN antibody
      • Minichromosome maintenance complex component 6 antibody
      • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
      • Minichromosome maintenance deficient 6 homolog antibody
      • Minichromosome maintenance protein 6 antibody
      • Mis5 antibody
      • MIS5 homolog antibody
      • p105MCM antibody
      see all

    图片

    • Western blot - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
      Western blot - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
      All lanes : Anti-MCM6 antibody [1/MCM6] (ab280208) at 1/1000 dilution

      Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
      Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
      Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
      Lane 5 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
      Lane 6 : Human tonsil tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 93 kDa



      This data was developed using ab280208 the same antibody clone in a different buffer formulation.

      Blocking and Diluting buffer and concentration: 5% NFDM/TBST

      Exposure time lane 1-5: 37 seconds
      lane 6: 3 minutes

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

      This data was developed using ab280208 the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MCM6 with ab280208 at 1/200 (4.945 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in human colon.The section was incubated with ab280208 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

      This data was developed using ab280208 the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MCM6 with ab280208 at 1/200 (4.945 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in human tonsil.The section was incubated with ab280208 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

      This data was developed using ab280208 the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling MCM6 with ab280208 at 1/20 (49.45 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in A431 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
      Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

      This data was developed using ab280208 the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS cells labelling MCM6 with ab280208 at 1/20 (49.45 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in U-2 OS cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
      Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab280215？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab280215 尚未被引用在任何文献中。

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