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RabMAb

Anti-MCM5抗体[EP2682Y] (ab76023)

Reviews (1) Submit a question References (2)
Western blot - Anti-MCM5 antibody [EP2682Y] (ab76023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

Key features and details

  • Rabbit monoclonal [EP2682Y] to MCM5
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-MCM5抗体[EP2682Y]
    参阅全部 MCM5 一抗

  • 描述

    兔单克隆抗体[EP2682Y] to MCM5

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, MCF7 and human spleen lysates IHC-P: human squamous cell cervical carcinoma tissue, normal tonsil tissue, Glioma tissue, Colonic adenocarcinoma tissue, Endometrial carcinoma tissue and Ovarian carcinoma tissue

  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP2682Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76023于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/2000. Predicted molecular weight: 82 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/2000. Predicted molecular weight: 82 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

靶标

  • 序列相似性

    Belongs to the MCM family.
    Contains 1 MCM domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CDC 46 antibody
      • CDC46 antibody
      • CDC46 homolog antibody
      • Cell division cycle 46 antibody
      • DNA replication licensing factor antibody
      • DNA replication licensing factor MCM 5 antibody
      • DNA replication licensing factor MCM5 antibody
      • MCM 5 antibody
      • MCM5 antibody
      • MCM5_HUMAN antibody
      • MGC5315 antibody
      • Minichromosome maintenance complex component 5 antibody
      • Minichromosome maintenance deficient (S. cerevisiae) 5 antibody
      • Minichromosome maintenance deficient 5 antibody
      • Minichromosome maintenance deficient protein 5 antibody
      • P1 CDC46 antibody
      • P1-CDC46 antibody
      see all

    图片

    • Western blot - Anti-MCM5 antibody [EP2682Y] (ab76023)
      Western blot - Anti-MCM5 antibody [EP2682Y] (ab76023)
      All lanes : Anti-MCM5 antibody [EP2682Y] (ab76023) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 82 kDa
      Observed band size: 82 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

      ab76023, at a 1/100 dilution, staining MCM5 in paraffin embedded human squamous cell cervical carcinoma tissue. A HRP/AP polymerized secondary antibody was used.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

      ab76023 showing positive staining in Normal tonsil tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

      ab76023 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

      ab76023 showing positive staining in Glioma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

      ab76023 showing positive staining in Colonic adenocarcinoma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

      ab76023 showing positive staining in Endometrial carcinoma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

      ab76023 showing positive staining in Ovarian carcinoma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    文献 (2)

    发表研究结果有使用 ab76023？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab76023 被引用在 2 文献中.

    • Zhang W  et al. NFIB facilitates replication licensing by acting as a genome organizer. Nat Commun 14:5076 (2023). PubMed: 37604829
    • Zhang R  et al. Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing. Oncotarget 7:80190-80207 (2016). Human . PubMed: 27863397

    客户评价及客户问答

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-MCM5 antibody [EP2682Y]

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    提交于 Jan 02 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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