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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other

Anti-MCM4抗体(ab3728)

  • Datasheet
  • SDS
Submit a review Submit a question References (7)

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Western blot - Anti-MCM4 antibody (ab3728)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)

Key features and details

  • Goat polyclonal to MCM4
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-MCM4抗体
    参阅全部 MCM4 一抗
  • 描述

    山羊多克隆抗体to MCM4
  • 宿主

    Goat
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 4 encoded in part by exons 8 and 9.

  • 阳性对照

    • WB: HeLa, HEK293T, Jurkat cell lysate. IHC-P: human ovarian carcinoma, mouse renal cell carcinoma.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3728于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
说明
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

靶标

  • 功能

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • 序列相似性

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession P33991 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4173 Human
    • Entrez Gene: 17217 Mouse
    • Omim: 602638 Human
    • SwissProt: P33991 Human
    • SwissProt: P49717 Mouse
    • Unigene: 460184 Human
    • Unigene: 1500 Mouse
    • 别名

      • 19G antibody
      • AI325074 antibody
      • AU045576 antibody
      • CDC 21 antibody
      • CDC 54 antibody
      • CDC21 antibody
      • CDC21 homolog antibody
      • CDC21, S. pombe, homolog of antibody
      • CDC54 antibody
      • Cell division cycle 21, S. pombe, homolog of antibody
      • DNA replication licensing factor MCM 4 antibody
      • DNA replication licensing factor MCM4 antibody
      • hCdc 21 antibody
      • hCdc21 antibody
      • Homolog of S. pombe cell devision cycle 21 antibody
      • KIAA4003 antibody
      • mcdc21 antibody
      • MCM 4 antibody
      • MCM 4 minichromosome maintenance deficient 4 antibody
      • mcm4 antibody
      • MCM4 minichromosome maintenance deficient 4 antibody
      • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
      • MCM4_HUMAN antibody
      • Mcmd4 antibody
      • MGC33310 antibody
      • Minichromosome maintenance 4 antibody
      • Minichromosome maintenance complex component 4 antibody
      • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
      • Minichromosome maintenance deficient 4 antibody
      • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
      • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
      • mKIAA4003 antibody
      • NKCD antibody
      • NKGCD antibody
      • P1 CDC21 antibody
      • P1-CDC21 antibody
      • P1CDC21 antibody
      see all

    图片

    • Western blot - Anti-MCM4 antibody (ab3728)
      Western blot - Anti-MCM4 antibody (ab3728)
      All lanes : Anti-MCM4 antibody (ab3728) at 1/10000 dilution

      Lane 1 : HeLa Whole cell lysate
      Lane 2 : HEK293T Whole cell lysate
      Lane 3 : Jurkat Whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 100 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1µg/ml). Detection: DAB.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1µg/ml). Detection: DAB.

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (7)

    发表研究结果有使用 ab3728?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3728 被引用在 7 文献中.

    • Ni H  et al. MiR-95-5p involves in the migration and invasion of trophoblast cells by targeting low density lipoprotein receptor-related protein 6. J Obstet Gynaecol Res 47:184-197 (2021). PubMed: 32924237
    • Liu H  et al. Downregulated miR-130a enhances the sensitivity of acute myeloid leukemia cells to Adriamycin. Mol Med Rep 22:2810-2816 (2020). PubMed: 32945422
    • Lv Q  et al. Pirfenidone alleviates pulmonary fibrosis in vitro and in vivo through regulating Wnt/GSK-3ß/ß-catenin and TGF-ß1/Smad2/3 signaling pathways. Mol Med 26:49 (2020). PubMed: 32448163
    • Zhang L  et al. LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR-346. Int J Mol Med 46:211-223 (2020). PubMed: 32319541
    • Tao Y  et al. MiR-214-3p regulates the viability, invasion, migration and EMT of TNBC cells by targeting ST6GAL1. Cytotechnology 71:1155-1165 (2019). PubMed: 31705333
    • Tenca P  et al. Cdc7 is an active kinase in human cancer cells undergoing replication stress. J Biol Chem 282:208-15 (2007). PubMed: 17062569
    • Hochegger H  et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB ; Human, Chicken . PubMed: 17635936

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