重组Anti-MCM3抗体[S04-6B9] (ab317372)
![Western blot - Anti-MCM3 Antibody [S04-6B9] (ab317372)](https://www.abcam.cn/ps/products/317/ab317372/Images/ab317372-536440-anti-mcm3-antibody-s04-6b9-western-blot-jurkat-c6-hela-human-rat.jpg "Western blot - Anti-MCM3 Antibody [S04-6B9] (ab317372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [S04-6B9] to MCM3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
概述
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产品名称
Anti-MCM3抗体[S04-6B9]
参阅全部 MCM3 一抗 -
描述
兔单克隆抗体[S04-6B9] to MCM3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human MCM3.
Database link: P25205 -
阳性对照
- WB: Jurkat, C6 and HeLa cell lysate. IHC-P: Human tonsil tissue. ICC/IF: HeLa cells.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
S04-6B9 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317372于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 91 kDa.
|
|
| ICC/IF |
1/20 - 1/50.
|
|
| IHC-P |
1/20 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 91 kDa. |
|
ICC/IF
1/20 - 1/50. |
|
IHC-P
1/20 - 1/100. |
靶标
-
功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4172 Human
- Entrez Gene: 316273 Rat
- Omim: 602693 Human
- SwissProt: P25205 Human
- Unigene: 179565 Human
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别名
- Cervical cancer proto oncogene 5 antibody
- DNA polymerase alpha holoenzyme associated P1 antibody
- DNA polymerase alpha holoenzyme associated protein P1 antibody
see all
图片
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Western blot - Anti-MCM3 Antibody [S04-6B9] (ab317372)All lanes : Anti-MCM3 Antibody [S04-6B9] (ab317372) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : C6 cell lysate
Lane 3 : HeLa cell lysate
Predicted band size: 91 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 Antibody [S04-6B9] (ab317372)](https://www.abcam.cn/ps/products/317/ab317372/Images/ab317372-536439-anti-mcm3-antibody-s04-6b9-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 Antibody [S04-6B9] (ab317372)Immunohistochemistry of MCM3 in paraffin-embedded human tonsil using ab317372 at dilution 1/1.
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![Immunocytochemistry/ Immunofluorescence - Anti-MCM3 Antibody [S04-6B9] (ab317372)](https://www.abcam.cn/ps/products/317/ab317372/Images/ab317372-536438-anti-mcm3-antibody-s04-6b9-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MCM3 Antibody [S04-6B9] (ab317372)Immunofluorescence of MCM3 (green) in HeLa cells using ab317372 at dilution 1/200, and DAPI (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317372 尚未被引用在任何文献中。