Anti-MCM3抗体(ab4460)
Key features and details
- Rabbit polyclonal to MCM3
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MCM3抗体
参阅全部 MCM3 一抗 -
描述
兔多克隆抗体to MCM3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IP, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Orangutan -
免疫原
Synthetic peptide within Human MCM3 aa 500-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_002379.2 (GeneID 4172).
Database link: P25205 -
阳性对照
- WB: HeLa cell RIPA extract. HeLa OHIO extract. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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IP |
Use at 2-10 µg/mg of lysate.
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WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 90 kDa).
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 90 kDa). |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4172 Human
- Entrez Gene: 17215 Mouse
- Omim: 602693 Human
- SwissProt: P25205 Human
- SwissProt: P25206 Mouse
- Unigene: 179565 Human
- Unigene: 4502 Mouse
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别名
- Cervical cancer proto oncogene 5 antibody
- DNA polymerase alpha holoenzyme associated P1 antibody
- DNA polymerase alpha holoenzyme associated protein P1 antibody
see all
图片
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Whole cell lysate (10 µg) from HeLa, Jurkat, HEK-293T, and TCMK-1 cells prepared using NETN lysis buffer.
ab4460 used for WB at 0.04 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling MCM3 with ab4460 at 1/1000 (1µg/ml). Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling MCM3 with ab4460 at 1/1000 (1µg/ml). Detection: DAB.
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Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lanes 1 and 2: ab4460 used for IP at 6 µg per reaction, two lots tested.
Lane 3 Control IgG.
For blotting immunoprecipitated MCM3, ab4460 was used at 0.04 µg/ml.
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HeLa cell RIPA extract (50
µ g) incubated with ab4460 used at 0.2µ g/ml for 1 hour. HeLa cell RIPA extract (50 µg) incubated with ab4460 used at 0.2 µg/ml for 1 hour. -
WB using ab4460. Western blot analysis of ab4460 on HeLa OHIO extract.
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ICC/IF image of ab4460 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4460, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab4460 被引用在 23 文献中.
- Drosopoulos WC et al. TRF2 Mediates Replication Initiation within Human Telomeres to Prevent Telomere Dysfunction. Cell Rep 33:108379 (2020). PubMed: 33176153
- Prikrylova T et al. 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication. Sci Rep 9:11065 (2019). PubMed: 31363131
- Yang Q et al. Minichromosome maintenance 3 promotes hepatocellular carcinoma radioresistance by activating the NF-?B pathway. J Exp Clin Cancer Res 38:263 (2019). PubMed: 31208444
- Zhang Y et al. Histone H3K27 methylation modulates the dynamics of FANCD2 on chromatin to facilitate NHEJ and genome stability. J Cell Sci 131:N/A (2018). PubMed: 29760279
- Charrasse S et al. Ensa controls S-phase length by modulating Treslin levels. Nat Commun 8:206 (2017). PubMed: 28785014