重组Anti-MCM2 (phospho S41)抗体[EPR4171] (ab109270)
![Western blot - Anti-MCM2 (phospho S41) antibody [EPR4171] (ab109270)](https://www.abcam.cn/ps/products/109/ab109270/Images/ab109270-122004-anti-mcm2-phospho-s41-antibody-epr4171-western-blot.jpg "Western blot - Anti-MCM2 (phospho S41) antibody [EPR4171] (ab109270)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4171] to MCM2 (phospho S41)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-MCM2 (phospho S41)抗体[EPR4171]
参阅全部 MCM2 一抗 -
描述
兔单克隆抗体[EPR4171] to MCM2 (phospho S41) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- HeLa cell lysate; Human tonsil tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4171 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109270于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50000 - 1/200000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/50000 - 1/200000. Detects a band of approximately 125 kDa (predicted molecular weight: 102 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt.
靶标
-
功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
-
别名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
图片
-
Western blot - Anti-MCM2 (phospho S41) antibody [EPR4171] (ab109270)All lanes : Anti-MCM2 (phospho S41) antibody [EPR4171] (ab109270) at 1/50000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with Alkaline Phosphatase (AP)
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S41) antibody [EPR4171] (ab109270)](https://www.abcam.cn/ps/products/109/ab109270/Images/ab109270-122003-anti-mcm2-phospho-s41-antibody-epr4171-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S41) antibody [EPR4171] (ab109270)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109270 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-MCM2 (phospho S41) antibody [EPR4171] (ab109270)](https://www.abcam.cn/ps/products/109/ab109270/Images/ab109270-3-benefits-of-recombinant-antibodies.png)
Anti-MCM2 (phospho S41) antibody [EPR4171] (ab109270)
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab109270 被引用在 1 文献中.
- Liu Y et al. Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome. Oncol Lett 21:117 (2021). PubMed: 33408763