重组Anti-MCM2抗体[RM1146] - BSA and Azide free (ab317753)
![Western blot - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-542037-anti-mcm2-antibody-rm1146-western-blot-hela-human-cervical-adenocarcinoma-epithelial-cell-whole-cell-lysate-20-ug.jpg "Western blot - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM1146] to MCM2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MCM2抗体[RM1146] - BSA and Azide free
参阅全部 MCM2 一抗 -
描述
兔重组multiclonal [RM1146] to MCM2 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
阳性对照
- WB: HeLa, 293T, NIH/3T3, PC-12, C6, Neuro-2a, U-87 MG, SH-SY5Y, NIH/3T3 transfected with siRNA specifically targeting MCM2 and 293T transfected with siRNA specifically targeting MCM2. IHC-P: Human colon, Human colon carcinoma, Mouse colon and Rat colon tissues. ICC/IF: HeLa, NIH/3T3 and C6 cells. Flow Cyt (Intra): NIH/3T3, HeLa and C6 cells.
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常规说明
ab317753 is the carrier-free version of ab317752
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
Recombinant Multiclonal -
克隆编号
RM1146 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317753于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 102 kDa.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 102 kDa. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
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别名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
图片
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Western blot - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)All lanes : Anti-MCM2 antibody [RM1146] (ab317752) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis data was developed using ab317752, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
-
![Western blot - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-542039-anti-mcm2-antibody-rm1146-western-blot-nih3t3-mouse-embryonic-fibroblast-transfected-with-scrambled-sirna-control-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)All lanes : Anti-MCM2 antibody [RM1146] (ab317752) at 1/1000 dilution
Lanes 1 & 5 : NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : NIH/3T3 transfected with siRNA specifically targeti MCM2, whole cell lysate
Lanes 3 & 7 : 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lanes 4 & 8 : 293T transfected with siRNA specifically targeting MCM2, whole cell lysate
Lane 6 : NIH/3T3 transfected with siRNA specifically targeting MCM2, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
Anti-MCM2 antibody (ab317752) staining at 1/1000 dilution showed similar expression profile/ molecular weight with Anti-MCM2 antibody (ab4461) at 1/1000 dilution.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-2: 6 seconds Lane 3-8: 10 seconds
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![Western blot - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-542038-anti-mcm2-antibody-rm1146-western-blot-c6-rat-glial-tumor-glial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)All lanes : Anti-MCM2 antibody [RM1146] (ab317752) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis data was developed using ab317752, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15210935 and PMID: 22123827).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-5-anti-mcm2-antibody-rm1146-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MCM2 with ab317752 at 1/4000 (0.127 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon. The section was incubated with ab317752 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-6-anti-mcm2-antibody-rm1146-immunohistochemistry-human-colon-carcinom.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling MCM2 with ab317752 at 1/4000 (0.127 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon carcinoma. The section was incubated with ab317752 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-8-anti-mcm2-antibody-rm1146-immunohistochemistry-rat-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MCM2 with ab317752 at 1/4000 (0.127 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat colon. The section was incubated with ab317752 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-7-anti-mcm2-antibody-rm1146-immunohistochemistry-mouse-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MCM2 with ab317752 at 1/4000 (0.127 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse colon. The section was incubated with ab317752 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-2-anti-mcm2-antibody-rm1146-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling MCM2 with ab317752 at 1/1000 (0.509 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing nuclear staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
-
![Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-3-anti-mcm2-antibody-rm1146-immunocytochemistry-nih3t3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling MCM2 with ab317752 at 1/1000 (0.509 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing nuclear staining in NIH/3T3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
-
![Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-4-anti-mcm2-antibody-rm1146-immunocytochemistry-c6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) cells labelling MCM2 with ab317752 at 1/1000 (0.509 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing nuclear staining in C6 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
-
![Flow Cytometry (Intracellular) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-9-anti-mcm2-antibody-rm1146-flow-cytometry-nih3t3.jpg)
Flow Cytometry (Intracellular) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling MCM2 with ab317752 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
-
![Flow Cytometry (Intracellular) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-10-anti-mcm2-antibody-rm1146-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling MCM2 with ab317752 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
-
![Flow Cytometry (Intracellular) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-11-anti-mcm2-antibody-rm1146-flow-cytometry-c6.jpg)
Flow Cytometry (Intracellular) - Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)This data was developed using ab317752, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labelling MCM2 with ab317752 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
-
![Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)](https://www.abcam.cn/ps/products/317/ab317753/Images/ab317753-1-BenefitsofRecombinantantibodies.jpg)
Anti-MCM2 antibody [RM1146] - BSA and Azide free (ab317753)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab317753 尚未被引用在任何文献中。
