AB108935

重组Anti-MCM2抗体[EPR4120]

Anti-MCM2 antibody [EPR4120]

RabMAb
Recombinant
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(36 Publications)

Anti-MCM2 antibody [EPR4120] (ab108935) is a rabbit monoclonal antibody detecting MCM2 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

查看别名

BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28

17 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)

Immunohistochemical staining of MCM2 in paraffin-embedded human tonsil tissue with unpurified ab108395 at a 1/250 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)

Unpurified ab108935 at a 1/100 dilution staining MCM2 in HeLa cells.

Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR4120] (AB108935)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR4120] (AB108935)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab108935 at 1/160 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)

Immunohistochemical staining of MCM2 in paraffin-embedded human squamous cell cervical carcinoma tissue with unpurified ab108395 at a 1/250 dilution.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab108935 at a dilution of 1/600. An Alexa Fluor^® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab108935 at a dilution of 1/250. An Alexa Fluor^® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)

Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling MCM2 with purified ab108935 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)

Immunohistochemical staining of paraffin embedded human tonsil with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)

Immunohistochemical staining of paraffin embedded rat liver with unpurified ab108935 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)

Immunohistochemical staining of paraffin embedded rat liver with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.

Lab

Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (AB108935)

MCM2 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab108935 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108935 at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/30 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate (Input) at 10 µg

Lane 2:

C6 (rat glial tumor glial cell) whole cell lysate (+)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 130 kDa

false

Exposure time: 3s

Lab

Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (AB108935)

MCM2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab108935 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108935 at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/30 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (Input) at 10 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (+)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 130 kDa

false

Exposure time: 3s

Unknown

Western blot - Anti-MCM2 antibody [EPR4120] (AB108935)

All lanes:

Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/10000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Lane 4:

Molt-4 cell lysate at 10 µg

Lane 5:

Jurkat cell lysate at 10 µg

Lane 6:

HeLa cell lysate at 10 µg

Predicted band size: 102 kDa

false

Lab

Western blot - Anti-MCM2 antibody [EPR4120] (AB108935)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 125 kDa

false

Lab

Western blot - Anti-MCM2 antibody [EPR4120] (AB108935)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 125 kDa

false

Lab

Western blot - Anti-MCM2 antibody [EPR4120] (AB108935)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 125 kDa

false

OI-RD Scanning - Anti-MCM2 antibody [EPR4120] (AB108935)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MCM2 antibody [EPR4120] (AB108935)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (12)

Carrier free

Anti-MCM2 antibody [EPR4120] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-MCM2 antibody [EPR4120]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-MCM2 antibody [EPR4120]
660 APC

APC Anti-MCM2 antibody [EPR4120]
HRP

HRP Anti-MCM2 antibody [EPR4120]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-MCM2 antibody [EPR4120]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-MCM2 antibody [EPR4120]
578 PE

PE Anti-MCM2 antibody [EPR4120]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-MCM2 antibody [EPR4120]
Carrier free

Anti-MCM2 antibody [RM1146] - BSA and Azide free
Unconjugated

Anti-MCM2 antibody [RM1146]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-MCM2 antibody [EPR4120]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4120

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "For unpurified, use 1/1000 - 1/5000.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "For unpurified, use 1/250.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "For unpurified, use 1/100." }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "For unpurified, use 1/1000 - 1/5000.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "For unpurified, use 1/100." } } }

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产品详情

What is this antibody validated in?
Anti-MCM2 antibody [EPR4120] (ab108935) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of MCM2?
Anti-MCM2 [EPR4120] (ab108935) specifically detects a band for MCM2 (UniProt: P49736) at a molecular weight of 102kDa.

Trusted by the scientific community
Anti-MCM2 [EPR4120] (ab108935) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR4120] also available for your convenience: ab108935, Alexa Fluor® 488 - ab223402, Alexa Fluor® 647 - ab223403, Carrier free - ab226044, PE - ab303154, APC - ab303155, HRP - ab303156, Alkaline Phosphatase - ab308707, Alexa Fluor® 594 - ab310426, Alexa Fluor® 555 - ab311952, Alexa Fluor® 568 - ab312425, Alexa Fluor® 750 - ab320919

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.

Biological function summary

MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.

Pathways

MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.

MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for the entry in S phase and for cell division (PubMed : 8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed : 26196677).

See full target information MCM2

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文献 (36)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:1506 PubMed41044692

2025

Expression and prognostic value of MCM2 in type B thymomas.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Du,Jian Cui,Xintao Yu,Dingfang Cao,Ying Zhang,Lei Yu,Shanqing Li

Cancers 17: PubMed40507244

2025

Protein Biomarkers Enable Sensitive and Specific Cervical Intraepithelial Neoplasia (CIN) II/III+ Detection: One Step Closer to Universal Cervical Cancer Screening.

Applications

Unspecified application

Species

Unspecified reactive species

Samrin F Habbani,Sayeh Dowlatshahi,Nathanael Lichti,Meaghan Broman,Lucy Tecle,Scott Bolton,Lisa Flowers,Rafael Guerrero-Preston,Jacqueline C Linnes,Sulma I Mohammed

Journal of Cancer 16:1348-1362 PubMed39895792

2025

EFHD1 Activates SIK3 to Limit Colorectal Cancer Initiation and Progression via the Hippo Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qionghui Huang,Xiaoyan Tang,Caiyan Gan,Qiaoting Deng,Shaobin Zhi,Qingyan Huang,Xiaoqi Zheng,Xueqiong Li,Zengfeng Pan,Mingfeng Huang

iScience 28:111745 PubMed39898029

2025

Nuclear talin-1 provides a bridge between cell adhesion and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro J Da Silva,Hendrik S E Hästbacka,Mikael C Puustinen,Jenny C Pessa,Jens C Luoto,Erika Sundström,Benjamin T Goult,Guillaume Jacquemet,Eva Henriksson,Lea Sistonen

Journal of molecular histology 56:24 PubMed39627566

2024

Intestinal mucosal turnover in germ-free piglets infected with E. coli.

Applications

Unspecified application

Species

Unspecified reactive species

Štefan Tóth,Zuzana Fagová,Monika Holodová,Kristína Čurgali,Eva Mechírová,Alexandra Kunová,Milan Maretta,Radomíra Nemcová,Soňa Gancarčíková,Marianna Danková

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403782 PubMed39412086

2024

CDC7 Inhibition Potentiates Antitumor Efficacy of PARP Inhibitor in Advanced Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shini Liu,Peng Deng,Zhaoliang Yu,Jing Han Hong,Jiuping Gao,Yulin Huang,Rong Xiao,Jiaxin Yin,Xian Zeng,Yichen Sun,Peili Wang,Ruizi Geng,Jason Yongsheng Chan,Peiyong Guan,Qiang Yu,Bin-Tean Teh,Qingping Jiang,Xiaojun Xia,Ying Xiong,Jianfeng Chen,Yongliang Huo,Jing Tan

Aging 16:11208-11223 PubMed39024509

2024

Cyclin L1 participates in Adriamycin resistance and progression of osteosarcoma via PI3K/AKT-mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbin Zhang,Tao Zhang,Long Chen,Zijun Guo,Xiaobing Jiang

Cell reports 43:114178 PubMed38703364

2024

Improved detection of DNA replication fork-associated proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca S Rivard,Ya-Chu Chang,Ryan L Ragland,Yee-Mon Thu,Muzaffer Kassab,Rahul Shubhra Mandal,Susan K Van Riper,Katarzyna Kulej,LeeAnn Higgins,Todd M Markowski,David Shang,Jack Hedberg,Luke Erber,Benjamin Garcia,Yue Chen,Anja-Katrin Bielinsky,Eric J Brown

Discover oncology 15:82 PubMed38512482

2024

FAM83D acts as an oncogene by regulating cell cycle progression via multiple pathways in synovial sarcoma: a potential novel downstream target oncogene of anlotinib.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Mei Liu,Ying Yuan,Lei Jin

Cell death & disease 15:129 PubMed38342917

2024

Deubiquitinase Mysm1 regulates neural stem cell proliferation and differentiation by controlling Id4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Xu,Qiaozhen Qin,Yan Wang,Heyang Zhang,Shuirong Liu,Xiaotong Li,Yue Chen,Yuqing Wang,Huaqiang Ruan,Wenyan He,Tao Zhang,Xinlong Yan,Changyong Wang,Donggang Xu,Xiaoxia Jiang

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