重组Anti-MCM2抗体[EPR3727] (ab133325)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3727] to MCM2
- Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MCM2抗体[EPR3727]
参阅全部 MCM2 一抗 -
描述
兔单克隆抗体[EPR3727] to MCM2 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, Jurkat, and MCF-7 cell lysates; Human cervical carcinoma and tonsil tissues; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3727 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133325于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/200.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/200. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4171 Human
- Omim: 116945 Human
- SwissProt: P49736 Human
- Unigene: 477481 Human
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别名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
图片
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All lanes : Anti-MCM2 antibody [EPR3727] (ab133325) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa -
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab133325 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling MCM2 with ab133325 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MCM2 with ab133325 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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MCM2 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) with ab133325 at 1/60 dilution.
Exposue time:10 second.
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Immunofluorescent analysis of HeLa cells labelling MCM2 with ab133325 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133325 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
Immunohistochemical analysis of paraffin embedded Human Clear cell carcinoma of kidney tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)
Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133325 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133325 被引用在 3 文献中.
- Liang Y et al. Protein kinase D1 phosphorylation of KAT7 enhances its protein stability and promotes replication licensing and cell proliferation. Cell Death Discov 6:89 (2020). PubMed: 33014433
- Chan YT et al. Concordant and discordant regulation of target genes by miR-31 and its isoforms. PLoS One 8:e58169 (2013). WB ; Human . PubMed: 23472152
- Ishikawa J et al. Suppressive effects of liquid crystal compounds on the growth of U937 human leukemic monocyte lymphoma cells. Cancer Cell Int 12:3 (2012). WB . PubMed: 22300067