Anti-MCM2抗体(ab3723)
Key features and details
- Goat polyclonal to MCM2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MCM2抗体
参阅全部 MCM2 一抗 -
描述
山羊多克隆抗体to MCM2 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human MCM2 aa 1-50.
Database link: NP_0045117.2 -
阳性对照
- HeLa cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa). |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
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别名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
图片
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Western blot - Anti-MCM2 antibody (ab3723)
Sample: Hela cell RIPA extract (60
µ g).Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000.
Detection: ECL.
Sample: Hela cell RIPA extract (60µg). Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000. Detection: ECL. -
Western blot - Anti-MCM2 antibody (ab3723)All lanes : Anti-MCM2 antibody (ab3723) at 0.5 µg/ml
Lane 1 : NIH3T3 cells
Lane 2 : TCMK-1 cells
Lane 3 : 4T1 cells
Lane 4 : CT26.WT cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 120 kDaDetection:Chemiluminescence with an exposed time of 3 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab3723 尚未被引用在任何文献中。