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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other

Anti-MCM2抗体(ab3723)

  • Datasheet
  • SDS
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Western blot - Anti-MCM2 antibody (ab3723)
  • Western blot - Anti-MCM2 antibody (ab3723)

Key features and details

  • Goat polyclonal to MCM2
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-MCM2抗体
    参阅全部 MCM2 一抗
  • 描述

    山羊多克隆抗体to MCM2
  • 宿主

    Goat
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human MCM2 aa 1-50.
    Database link: NP_0045117.2

  • 阳性对照

    • HeLa cell lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.00
    Preservative: 0.09% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3723于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).
说明
WB
1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).

靶标

  • 功能

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • 序列相似性

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 翻译后修饰

    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession P49736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4171 Human
    • Entrez Gene: 17216 Mouse
    • Omim: 116945 Human
    • SwissProt: P49736 Human
    • SwissProt: P97310 Mouse
    • Unigene: 477481 Human
    • Unigene: 16711 Mouse
    • 别名

      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    图片

    • Western blot - Anti-MCM2 antibody (ab3723)
      Western blot - Anti-MCM2 antibody (ab3723)

      Sample: Hela cell RIPA extract (60µg).

      Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000.

      Detection: ECL.

      Sample: Hela cell RIPA extract (60µg). Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000. Detection: ECL.
    • Western blot - Anti-MCM2 antibody (ab3723)
      Western blot - Anti-MCM2 antibody (ab3723)
      All lanes : Anti-MCM2 antibody (ab3723) at 0.5 µg/ml

      Lane 1 : NIH3T3 cells
      Lane 2 : TCMK-1 cells
      Lane 3 : 4T1 cells
      Lane 4 : CT26.WT cells

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 120 kDa



      Detection:Chemiluminescence with an exposed time of 3 minutes.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab3723?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3723 尚未被引用在任何文献中。

    客户评价及客户问答

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    提交评价 提交问题

    Immunohistochemistry (Frozen sections) abreview for Anti-MCM2 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse Embryo (day 8.5))
    Specification
    Mouse Embryo (day 8.5)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Saponin
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Derek Passer

    Verified customer

    提交于 Aug 26 2011

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