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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other

Anti-MCM10抗体(ab3733)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Western blot - Anti-MCM10 antibody (ab3733)

    Key features and details

    • Rabbit polyclonal to MCM10
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-MCM10抗体
      参阅全部 MCM10 一抗
    • 描述

      兔多克隆抗体to MCM10
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Chimpanzee, Rhesus monkey, Orangutan不与反应: Xenopus laevis, Drosophila melanogaster
    • 免疫原

      Synthetic peptide corresponding to Human MCM10.
      Database link: Q7L590

    • 阳性对照

      • WB: HeLa, HEK-293T and Jurkat whole cell lysates.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Synthesis
      • Other

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3733于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
    说明
    WB
    1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

    靶标

    • 功能

      Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication.
    • 序列相似性

      Belongs to the MCM10 family.
    • 发展阶段

      Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase.
    • 结构域

      The zinc finger-like domain binds a zinc ion and is involved in self-association.
    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位

      Nucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
    • Target information above from: UniProt accession Q7L590 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 55388 Human
      • Entrez Gene: 70024 Mouse
      • Omim: 609357 Human
      • SwissProt: Q3MIR3 Human
      • SwissProt: Q5T670 Human
      • SwissProt: Q7L590 Human
      • SwissProt: Q9H0D7 Human
      • SwissProt: Q9H3P9 Human
      • SwissProt: Q0VBD2 Mouse
      • SwissProt: Q505F3 Mouse
      • SwissProt: Q8BMJ1 Mouse
      • SwissProt: Q8BY66 Mouse
      • Unigene: 198363 Human
      • Unigene: 23828 Mouse
      • Unigene: 447935 Mouse
      see all
    • 别名

      • CNA43 antibody
      • DNA43 antibody
      • FLJ31133 antibody
      • Homolog of yeast MCM10 antibody
      • HsMCM10 antibody
      • mcm10 antibody
      • MCM10 minichromosome maintenance deficient 10 antibody
      • MCM10_HUMAN antibody
      • Minichromosome maintenance 10 replication initiation factor antibody
      • Minichromosome maintenance complex component 10 antibody
      • Minichromosome maintenance protein 10 isoform 1 antibody
      • PRO2249 antibody
      • Protein MCM10 homolog antibody
      see all

    图片

    • Western blot - Anti-MCM10 antibody (ab3733)
      Western blot - Anti-MCM10 antibody (ab3733)
      All lanes : Anti-MCM10 antibody (ab3733) at 1 µg/ml

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 110 kDa


      Exposure time: 3 minutes

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (7)

    发表研究结果有使用 ab3733?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3733 被引用在 7 文献中.

    • Tian J  et al. Aberrant MCM10 SUMOylation induces genomic instability mediated by a genetic variant associated with survival of esophageal squamous cell carcinoma. Clin Transl Med 11:e485 (2021). PubMed: 34185429
    • Guarino Almeida E  et al. A kinase-independent function for AURORA-A in replisome assembly during DNA replication initiation. Nucleic Acids Res 48:7844-7855 (2020). PubMed: 32652013
    • Wang M  et al. Minichromosome maintenance protein 10 as a marker for proliferation and prognosis in lung cancer. Int J Oncol 55:1349-1360 (2019). PubMed: 31638210
    • Ma Y  et al. Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. Oncotarget 8:104813-104830 (2017). PubMed: 29285216
    • Liao YX  et al. Silencing of RTKN2 by siRNA suppresses proliferation, and induces G1 arrest and apoptosis in human bladder cancer cells. Mol Med Rep 13:4872-8 (2016). PubMed: 27082503
    • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . PubMed: 24726359
    • Im JS  et al. Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci U S A 106:15628-32 (2009). PubMed: 19805216

    客户评价及客户问答

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    Western blot abreview for Anti-MCM10 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells and BJ human fibroblast cells)
    Loading amount
    20 µg
    Specification
    HeLa cells and BJ human fibroblast cells
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 03 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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