AB3733

Anti-MCM10 抗体

Name: MCM10抗体 (ab3733)| Abcam中文官网
Brand: Abcam Limited
SKU: AB3733
Price: 4606 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-MCM10 antibody

(1 Review)

(7 Publications)

Rabbit Polyclonal MCM10 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MCM10.

查看别名

PRO2249, MCM10, Protein MCM10 homolog, HsMCM10

1 Images

Supplier Data

Western blot - Anti-MCM10 antibody (AB3733)

All lanes:

Western blot - Anti-MCM10 antibody (ab3733) at 1 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 98 kDa

false

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human MCM10. The exact immunogen used to generate this antibody is proprietary information.

Q7L590

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCM10 also known as Mini-Chromosome Maintenance 10 is a component of the DNA replication machinery. This protein has an approximate molecular mass of 98 kDa. It functions mainly in the nucleus and is expressed across various cell types. MCM10 interacts closely with other replication factors to ensure DNA replication progression. Its mechanical role involves stabilizing the replication fork and binding to the replication origins.

Biological function summary

MCM10 plays an important role in the initiation and elongation phases of DNA replication. It acts as a scaffold for the assembly of other replication machinery components and facilitates the recruitment of DNA polymerase alpha. MCM10 is part of the larger pre-replication complex which includes other essential proteins such as MCM2-7 Cdc45 and GINS complex. These interactions ensure accurate DNA replication and cell cycle progression.

Pathways

MCM10 integrates into the DNA replication and repair pathways. Its function is critical in maintaining DNA integrity during the S phase of the cell cycle. In the DNA replication pathway MCM10's interaction with proteins like MCM2-7 and Cdc45 ensures the formation of replication forks. In the DNA damage response pathway it also plays a role by stabilizing the replication fork under stress conditions working alongside proteins like ATM and ATR to prevent replication stress.

MCM10 has links to cancer and genomic instability disorders. Abnormal expression levels of MCM10 can contribute to increased replication stress and genomic instability factors often seen in cancerous cells. MCM10 interacts with proteins like Cdt1 and Cdc6 whose dysregulation can lead to over-replication and cancer progression. Understanding MCM10’s role in these processes can provide insights into potential therapeutic interventions.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38 (PubMed : 24726359).

See full target information MCM10

style

left-column

文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 11:e485 PubMed34185429

2021

Aberrant MCM10 SUMOylation induces genomic instability mediated by a genetic variant associated with survival of esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbo Tian,Zequn Lu,Siyuan Niu,Shanshan Zhang,Pingting Ying,Lu Wang,Ming Zhang,Yimin Cai,Tianyi Dong,Ying Zhu,Rong Zhong,Zhihua Wang,Jiang Chang,Xiaoping Miao

Nucleic acids research 48:7844-7855 PubMed32652013

2020

A kinase-independent function for AURORA-A in replisome assembly during DNA replication initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Estrella Guarino Almeida,Xavier Renaudin,Ashok R Venkitaraman

International journal of oncology 55:1349-1360 PubMed31638210

2019

Minichromosome maintenance protein 10 as a marker for proliferation and prognosis in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,Songping Xie,Wen Yuan,Tian Xie,Muhammad Jamal,Jie Huang,Qian Yin,Hengya Song,Qiuping Zhang

Oncotarget 8:104813-104830 PubMed29285216

2017

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yinan Ma,Liangru Fei,Meiyu Zhang,Wenzhu Zhang,Xiaofang Liu,Congcong Wang,Yuan Luo,Haiyan Zhang,Yuchen Han

Molecular medicine reports 13:4872-8 PubMed27082503

2016

Silencing of RTKN2 by siRNA suppresses proliferation, and induces G1 arrest and apoptosis in human bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Xiang Liao,Jin-Min Zeng,Jia-Jie Zhou,Guang-Hua Yang,Kun Ding,Xian-Jue Zhang

Cell reports 7:575-587 PubMed24726359

2014

The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability.

Applications

Species

Unspecified reactive species

Benoit Miotto,Moredreck Chibi,Ping Xie,Stéphane Koundrioukoff,Hanlie Moolman-Smook,David Pugh,Michelle Debatisse,Fuchu He,Lingqiang Zhang,Pierre-Antoine Defossez

Proceedings of the National Academy of Sciences of 106:15628-32 PubMed19805216

2009

Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Sub Im,Sang-Hee Ki,Andrea Farina,Dong-Soo Jung,Jerard Hurwitz,Joon-Kyu Lee

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM10 抗体

Anti-MCM10 antibody

Western blot - Anti-MCM10 antibody (AB3733)