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Anti-MCL1 (phospho S159)抗体(ab111574)

Reviews (1)Q&A (3)References (5)
Immunocytochemistry/ Immunofluorescence - Anti-MCL1 (phospho S159) antibody (ab111574)

    Key features and details

    • Rabbit polyclonal to MCL1 (phospho S159)
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-MCL1 (phospho S159)抗体
      参阅全部 MCL1 一抗

    • 描述

      兔多克隆抗体to MCL1 (phospho S159)

    • 宿主

      Rabbit

    • 经测试应用

      适用于: ICC/IFmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide corresponding to Human MCL1 (phospho S159).

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab111574于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    1/100 - 1/500.
    说明
    ICC/IF
    1/100 - 1/500.

    靶标

    • 功能

      Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

    • 序列相似性

      Belongs to the Bcl-2 family.

    • 翻译后修饰

      Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
      Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
      Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.

    • 细胞定位

      Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Bcl 2 related protein EAT/mcl1 antibody
        • Bcl-2-like protein 3 antibody
        • Bcl-2-related protein EAT/mcl1 antibody
        • BCL2 related antibody
        • Bcl2-L-3 antibody
        • BCL2L3 antibody
        • EAT antibody
        • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
        • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
        • MCL 1 antibody
        • MCL1 antibody
        • MCL1-ES antibody
        • mcl1/EAT antibody
        • MCL1_HUMAN antibody
        • MCL1L antibody
        • MCL1S antibody
        • MGC104264 antibody
        • MGC1839 antibody
        • Myeloid Cell Leukemia 1 antibody
        • Myeloid cell leukemia ES antibody
        • Myeloid cell leukemia sequence 1 antibody
        • Myeloid cell leukemia sequence 1 BCL2 related antibody
        • Myeloid cell leukemia sequence 1 isoform 1 antibody
        • OTTHUMP00000032794 antibody
        • OTTHUMP00000032795 antibody
        • TM antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 (phospho S159) antibody (ab111574)
        Immunocytochemistry/ Immunofluorescence - Anti-MCL1 (phospho S159) antibody (ab111574)
        ab111574, at 1/100 dilution, staining MCL1 in A549 cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunising peptide.

      文献 (5)

      发表研究结果有使用 ab111574？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab111574 被引用在 5 文献中.

      • Yu X  et al. Skp2 stabilizes Mcl-1 and confers radioresistance in colorectal cancer. Cell Death Dis 13:249 (2022). PubMed: 35301297
      • Gao F  et al. Deguelin suppresses non-small cell lung cancer by inhibiting EGFR signaling and promoting GSK3ß/FBW7-mediated Mcl-1 destabilization. Cell Death Dis 11:143 (2020). PubMed: 32081857
      • Sarabhai T  et al. Serum a-1 Antitrypsin (AAT) antagonizes intrinsic apoptosis induction in neutrophils from patients with systemic inflammatory response syndrome. PLoS One 12:e0177450 (2017). WB . PubMed: 28493974
      • Pan R  et al. Synthetic Lethality of Combined Bcl-2 Inhibition and p53 Activation in AML: Mechanisms and Superior Antileukemic Efficacy. Cancer Cell 32:748-760.e6 (2017). PubMed: 29232553
      • Weigel KJ  et al. CAF-secreted IGFBPs regulate breast cancer cell anoikis. Mol Cancer Res 12:855-66 (2014). PubMed: 24803643

      客户评价及客户问答

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      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-MCL1 (phospho S159) antibody

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Liver cells)
      Loading amount
      80 µg
      Specification
      Liver cells
      Treatment
      DMSO 24 h or tBHQ different time periods
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 05 2012

      Question

      I have submitted abreview but please change fig. with current attached figures.

      Read More
      Answer

      Thank you for contacting us.

      I have replaced the image with the one you just sent me, and copied the figure legend into the Additional Notes box on the Abreview. The review will be published shortly.

      Thank you for testing the antibody in WB and for submitting your review.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      will test ab111574 in WB

      Read More
      Answer

      Thank you for your phone call.

      DISCOUNT CODE: xxx
      Expiration date: xxx

      I am very pleased to hear you would like to accept our offer and test ab111574 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

      Read More

      Question

      Have you tested its reactivity with Mcl-1(pSer159/pThr163)?

      Read More
      Answer

      I have confirmed that the antibody ab111574, raised against MCL1 pSer159, has not been tested for reactivity with MCL1 pSer159/pThr163.

      Read More

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