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Cell Biology Apoptosis Intracellular Bcl2 Family
使用敲除细胞株进行验证重组RabMAb

重组Anti-MCL1抗体[Y37] (ab32087)

  • Datasheet
  • SDS
Reviews (14)Q&A (6)References (118)

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Western blot - Anti-MCL1 antibody [Y37] (ab32087)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody [Y37] (ab32087)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
  • Flow Cytometry (Intracellular) - Anti-MCL1 antibody [Y37] (ab32087)
  • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
  • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
  • Flow Cytometry (Intracellular) - Anti-MCL1 antibody [Y37] (ab32087)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
  • Anti-MCL1 antibody [Y37] (ab32087)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y37] to MCL1
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

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概述

  • 产品名称

    Anti-MCL1抗体[Y37]
    参阅全部 MCL1 一抗
  • 描述

    兔单克隆抗体[Y37] to MCL1
  • 宿主

    Rabbit
  • 特异性

    ab32087 recognises MCL1. The antibody does not cross-react with other Bcl-2 family members.
  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human MCL1 aa 100-200. The exact sequence is proprietary.
    Database link: Q07820
    (Peptide available as ab199979)

  • 阳性对照

    • WB: Human lung, lung cancer and liver lysates; HEK293T, A431, Ramos, H:-60, HeLa, MCF7 and HepG2 whole cell lysate (ab7900). IHC-P: Human colon adenocarcinoma tissue. ICC/IF: HCT 116, MCF7 and H1299 cells. Flow Cyt (intra): Ramos and A431 cells.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    Y37
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

相关产品

  • Alternative Versions

    • Anti-MCL1 antibody [Y37] - BSA and Azide free (ab186822)
    • Alexa Fluor® 647 Anti-MCL1 antibody [Y37] (ab197035)
    • Alexa Fluor® 488 Anti-MCL1 antibody [Y37] (ab197529)
    • PE Anti-MCL1 antibody [Y37] (ab209289)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human MCL1 knockout HEK-293T cell line (ab266838)
  • KO cell lysates

    • Human MCL1 knockout HEK-293T cell lysate (ab256986)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human MCL1 protein (ab131682)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab32087于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/250.

For unpurified use 1 ug for 106 cells. (For lot-specific stock concentration, please contact Abcam).ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (2)
1/100 - 1/500.
WB (10)
1/1000 - 1/5000. Predicted molecular weight: 37 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IP (1)
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
1/250.

For unpurified use 1 ug for 106 cells. (For lot-specific stock concentration, please contact Abcam).ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100 - 1/500.
WB
1/1000 - 1/5000. Predicted molecular weight: 37 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IP
Use at an assay dependent concentration.

靶标

  • 功能

    Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • 序列相似性

    Belongs to the Bcl-2 family.
  • 翻译后修饰

    Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • 细胞定位

    Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
  • Target information above from: UniProt accession Q07820 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4170 Human
    • Entrez Gene: 17210 Mouse
    • Entrez Gene: 60430 Rat
    • Omim: 159552 Human
    • SwissProt: Q07820 Human
    • SwissProt: P97287 Mouse
    • SwissProt: Q9Z1P3 Rat
    • Unigene: 632486 Human
    • 别名

      • Bcl 2 related protein EAT/mcl1 antibody
      • Bcl-2-like protein 3 antibody
      • Bcl-2-related protein EAT/mcl1 antibody
      • BCL2 related antibody
      • Bcl2-L-3 antibody
      • BCL2L3 antibody
      • EAT antibody
      • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
      • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
      • MCL 1 antibody
      • MCL1 antibody
      • MCL1-ES antibody
      • mcl1/EAT antibody
      • MCL1_HUMAN antibody
      • MCL1L antibody
      • MCL1S antibody
      • MGC104264 antibody
      • MGC1839 antibody
      • Myeloid Cell Leukemia 1 antibody
      • Myeloid cell leukemia ES antibody
      • Myeloid cell leukemia sequence 1 antibody
      • Myeloid cell leukemia sequence 1 BCL2 related antibody
      • Myeloid cell leukemia sequence 1 isoform 1 antibody
      • OTTHUMP00000032794 antibody
      • OTTHUMP00000032795 antibody
      • TM antibody
      see all

    图片

    • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
      Western blot - Anti-MCL1 antibody [Y37] (ab32087)
      All lanes : Anti-MCL1 antibody [Y37] (ab32087) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : MCL1 knockout HEK293T cell lysate
      Lane 3 : Ramos cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 37 kDa
      Observed band size: 37 kDa



      Lanes 1-3: Merged signal (red and green). Green - ab32087 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa. 

       ab32087  Anti-MCL1 antibody [Y37]  was shown to specifically react with MCL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266838 (knockout cell lysate ab256986) was used.  Wild-type and MCL1 knockout samples were subjected to SDS-PAGE.  ab32087 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody [Y37] (ab32087)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody [Y37] (ab32087)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling MCL1 with purified ab32087 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
    • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
      Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)

      Immunocytochemistry/Immunofluorescence analysis of HCT 116 (human colorectal carcinoma cell line) cells labelling MCL1 with purified ab32087 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Flow Cytometry (Intracellular) - Anti-MCL1 antibody [Y37] (ab32087)
      Flow Cytometry (Intracellular) - Anti-MCL1 antibody [Y37] (ab32087)

      Intracellular Flow Cytometry analysis ofRamos (human Burkitt's lymphoma cell line) cells labelling MCL1 with purified ab32087 at 1/250 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

       

       

    • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
      Western blot - Anti-MCL1 antibody [Y37] (ab32087)
      All lanes : Anti-MCL1 antibody [Y37] (ab32087) at 1/1000 dilution

      Lane 1 : Human lung tissue with NFDM/TBST
      Lane 2 : Human lung cancer tissue with NFDM/TBST
      Lane 3 : Human liver tissue with NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated. at 1/1000 dilution

      Predicted band size: 37 kDa



      Exposure time for samples 1-3: 15 seconds; exposure time for samples 4-6: 1 minute.

      Additional bands: We are unsure as to the identity of these extra bands.

    • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
      Western blot - Anti-MCL1 antibody [Y37] (ab32087)
      All lanes : Anti-MCL1 antibody [Y37] (ab32087) at 1/1000 dilution

      Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate with NFDM/TBST
      Lane 2 : HL-60 (human promyelocytic leukemia cell line) cell lysate with NFDM/TBST
      Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate with NFDM/TBST
      Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate with NFDM/TBST
      Lane 5 : MCF7 (human breast adenocarcinoma cell line) cell lysate with NFDM/TBST
      Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate with NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG (H+L), peroxidase conjugated. at 1/1000 dilution

      Predicted band size: 37 kDa


      Exposure time: 15 seconds


      Additional bands: We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
      Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)This image is courtesy of an anonymous Abreview.

      Immunocytochemistry/Immunofluorescence analysis of H1299 cells labelling MCL1 with unpurified ab32087. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% Serum for 1 hour at 24°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 24°C. The secondary antibody was an Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/2000. DAPI (blue) was used as the nuclear counterstain.

      See Abreview

    • Flow Cytometry (Intracellular) - Anti-MCL1 antibody [Y37] (ab32087)
      Flow Cytometry (Intracellular) - Anti-MCL1 antibody [Y37] (ab32087)

      Intracellular Flow Cytometry analysis ofA431 (human epidermoid carcinoma cell line) cells labelling MCL1 with unpurified ab32087 (red line). Cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32087, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. 

      Black - Isotype control, rabbit monoclonal IgG. 

      Acquisition of >5,000 events was performed. This antibody gave a decreased signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
      Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)

      Immunocytochemistry/Immunofluorescence analysis of HCT 116 (human colorectal carcinoma cell line) cells treated with wogonin (ab142471) labelling MCL1 with unpurified ab32087. Decrease of MCL1 expression correlates with increased concentration of wogonin, as described in literature. Cells were incubated at 37°C for 2h in media containing different concentrations of ab142471 (wogonin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32087 (1/100) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    • Anti-MCL1 antibody [Y37] (ab32087)
      Anti-MCL1 antibody [Y37] (ab32087)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (118)

    发表研究结果有使用 ab32087?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab32087 被引用在 118 文献中.

    • Zhang M  et al. Melatonin sensitizes esophageal cancer cells to 5-fluorouracil via promotion of apoptosis by regulating EZH2 expression. Oncol Rep 45:N/A (2021). PubMed: 33649858
    • Ren Y  et al. Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis. Front Pharmacol 12:560903 (2021). PubMed: 33841136
    • Wu M  et al. FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival. Am J Cancer Res 11:712-728 (2021). PubMed: 33791149
    • Zeng M  et al. In vitro Non-Small Cell Lung Cancer Inhibitory Effect by New Diphenylethane Isolated From Stems and Leaves of Dioscorea oppositifolia L. via ERß-STAT3 Pathway. Front Pharmacol 12:622681 (2021). PubMed: 33708130
    • Jiao D  et al. Bidirectional differentiation of BMSCs induced by a biomimetic procallus based on a gelatin-reduced graphene oxide reinforced hydrogel for rapid bone regeneration. Bioact Mater 6:2011-2028 (2021). PubMed: 33426373
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 20 Abreviews or Q&A

    Western blot abreview for Anti-MCL1 antibody [Y37]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (MDCK cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    MDCK cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 01 2022

    Western blot abreview for Anti-MCL1 antibody [Y37]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (3611-RF cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    3611-RF cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 01 2022

    Western blot abreview for Anti-MCL1 antibody [Y37]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    MEFs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 01 2022

    Western blot abreview for Anti-MCL1 antibody [Y37]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MIA PaCa-2 cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    MIA PaCa-2 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 01 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCL1 antibody [Y37]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas wt, KRAS driven PanIN,PDAC in mouse pancr)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: i tried citrate and tris-EDTA
    Permeabilization
    Yes - 0.1% triton
    Specification
    pancreas wt, KRAS driven PanIN,PDAC in mouse pancr
    Blocking step
    casblock (008120)- READY TO USE as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 10 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MCL1 antibody [Y37]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (GBM primary culture)
    Permeabilization
    Yes - PBST 0.3%
    Specification
    GBM primary culture
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Matthias Dedobbeleer

    Verified customer

    提交于 Sep 12 2018

    Western blot abreview for Anti-MCL1 antibody [Y37]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U87MG cells)
    Gel Running Conditions
    Non-reduced Denaturing (12)
    Loading amount
    50 µg
    Treatment
    10Gray Irradiation
    Specification
    U87MG cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Matthias Dedobbeleer

    Verified customer

    提交于 Jul 18 2018

    Question

    Inquiry: Dear Tech-team, does the antibody will detect mcl-1 which is complexed with bim,NOXA, bid or others?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 10 2016

    Answer




    I can confirm that we have not tested if this antibody is also binding to MCL1 in complexes.


    I would like to point out that the clone [Y37] (parent product ab32087) is tested for IP. We have customer feedback where this clone was used to co-IP MCL1 and then BAK was detected in the western blot which indicates that clone [Y37] also binds to MCL1 in complexes:


    https://www.abcam.com/MCL1-antibody-Y37-ab32087/reviews/21619


    Please note that this has not been tested by us and that it is not covered by our Abpromise guarantee.


    Read More

    Anja Hoffmann

    Abcam Scientific Support

    回复于 Mar 10 2016

    Question

    Please confirm which isotype are detected by ab32087

    Read More

    Abcam community

    Verified customer

    Asked on Apr 29 2013

    Answer

    I can confirm that the immunogen sequence for ab32087 has high alignment to both isoforms 1 and 2 from the following Uniprot protein database page for the protein:
    http://www.uniprot.org/uniprot/Q07820

    It is therefore very likely to detect both isoforms. however, i am sorry this has not been specifically tested in the laboratory.

    Read More

    Abcam Scientific Support

    回复于 Apr 29 2013

    Question

    Lot number: GR87086-2
    Inquiry: Dear Abcam, Can you give me information about antibody ab 32087 concentration in lot GR87086-2? Sincerely,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 28 2013

    Answer

    Thank you for contacting us.

    The concentration of lot GR87086-2 is 0.81 mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jan 28 2013

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