AB246684

Anti-MCL1抗体

Name: MCL1抗体 (ab246684)| Abcam中文官网
Brand: Abcam Limited
SKU: AB246684
Price: 4863 CNY
Availability: InStock

Anti-MCL1 antibody

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(3 Publications)

Rabbit Polyclonal MCL1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MCL1 aa 50-150.

查看别名

BCL2L3, MCL1, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, Bcl2-L-3

2 Images

Supplier Data

Immunoprecipitation - Anti-MCL1 antibody (AB246684)

MCL1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab246684 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab246684 at 1 μg/ml.

Lane 1 : ab246684 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-MCL1 antibody (ab246684)

Predicted band size: 37 kDa

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Supplier Data

Western blot - Anti-MCL1 antibody (AB246684)

All lanes:

Western blot - Anti-MCL1 antibody (ab246684) at 0.04 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HEK-293T whole cell lysate at 15 µg

Lane 3:

HEK-293T whole cell lysate at 5 µg

Predicted band size: 37 kDa

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Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human MCL1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q07820

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab246684 was affinity purified using an epitope specific to MCL1 immobilized on solid support.

存储溶液

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCL1 also known as myeloid cell leukemia sequence 1 is an anti-apoptotic member of the BCL-2 family. This protein plays an important role in regulating cell survival by inhibiting apoptosis. MCL1 has a molecular weight of approximately 37 kDa. It is primarily expressed in the mitochondria and can be found in various tissues including heart liver placenta and kidneys.

Biological function summary

MCL1 protein functions to maintain cell viability by counteracting pro-apoptotic stimuli. It interacts within the cell as part of a complex network involving other BCL-2 family proteins. MCL1 binds and sequesters BH3-only proteins and Bax therefore preventing them from initiating the apoptotic cascade. This interaction helps cells survive under stress conditions by temporarily inhibiting apoptosis.

Pathways

MCL1 plays a significant role in the intrinsic (mitochondrial) apoptosis pathway and the cell signaling pathways that facilitate cell survival and proliferation. In the mitochondrial pathway it works closely with other BCL-2 family members like BCL-xL and BCL-2 itself to control the permeability of the mitochondrial outer membrane and thereby influence cytochrome c release. The PI3K/AKT pathway can also regulate MCL1 leading to increases in its expression and stability which promotes cell survival.

Researchers have linked alterations in MCL1 expression to various cancers including leukemia and lymphoma. Overexpression of MCL1 can contribute to chemoresistance in cancer cells by preventing apoptosis. This protein's function is also connected to cardiomyopathies where altered MCL1 expression in cardiac tissue can influence cell death and survival outcomes. BCL-2 and BCL-xL closely related proteins also implicate in these conditions highlighting the central role of the apoptotic regulatory network in disease development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

See full target information MCL1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Dentistry journal 12: PubMed39452455

2024

NTRK Gene Expression Analysis in Oral Squamous Cell Carcinoma Mexican Population.

Applications

Unspecified application

Species

Unspecified reactive species

Lilibeth Stephania Escoto-Vasquez,Javier Portilla-Robertson,Josué Orlando Ramírez-Jarquín,Luis Fernando Jacinto-Alemán,Alejandro Alonso-Moctezuma,Carla Monserrat Ramírez-Martínez,Osmar Alejandro Chanes-Cuevas,Fabiola Salgado-Chavarria

Discover. Oncology 14:28 PubMed36853387

2023

Estrogen-dependent activation of NCOA3 couples with p300 and NF-κB to mediate antiapoptotic genes in ER-positive breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Zhiyong Zhou

Molecular medicine reports 23: PubMed33300066

2020

Long non‑coding RNA SNHG14 affects the proliferation and apoptosis of childhood acute myeloid leukaemia cells by modulating the miR‑193b‑3p/MCL1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Wang,Wenrong Li,Yanhong Chen,Lijuan Zhou

View all publications

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