AB316978

重组Anti-mCherry抗体[EPR20579] -鸡IgY (Chimeric) - BSA and Azide free

Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) - BSA and Azide free

Recombinant
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Chicken Recombinant Monoclonal mCherry antibody. Carrier free. Suitable for WB and reacts with Tag samples.

1 Images

Supplier Data

Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) - BSA and Azide free (AB316978)

This data was developed using ab316977, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) (<a href='/products/primary-antibodies/mcherry-antibody-epr20579-chicken-igy-chimeric-ab316977'>ab316977</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag® whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

293T transfected with mCherry expression vector containing a myc-His-tag® whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/20000 dilution

Observed band size: 27 kDa,36 kDa,35 kDa

false

Exposure time: 10s

不同偶联物与剂型 (3)

Unconjugated

Anti-mCherry antibody [EPR20579]
Carrier free

Anti-mCherry antibody [EPR20579] - BSA and Azide free
Unconjugated

Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric)

关键信息

宿主种属

Chicken

克隆

Monoclonal

克隆号

EPR20579

亚型

IgY

不含载体蛋白

Yes

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }

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产品详情

This chicken monoclonal chimeric antibody (ab316978) is the carrier-free version of ab316977.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Thiophilic Resin

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information mCherry

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-mCherry抗体[EPR20579] -鸡IgY (Chimeric) - BSA and Azide free