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AB316977

重组Anti-mCherry抗体[EPR20579] -鸡IgY (Chimeric)

Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric)

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Chicken Recombinant Monoclonal mCherry antibody. Suitable for WB and reacts with Tag samples.
1 Images
Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) (AB316977)
  • WB

Supplier Data

Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) (AB316977)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) (ab316977) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag® whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

293T transfected with mCherry expression vector containing a myc-His-tag® whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/20000 dilution

Observed band size: 27 kDa,36 kDa,35 kDa

false

Exposure time: 10s

不同偶联物与剂型 (3)

  • Carrier free

    Anti-mCherry antibody [EPR20579] - BSA and Azide free

  • Unconjugated

    Anti-mCherry antibody [EPR20579]

  • Carrier free

    Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) - BSA and Azide free

关键信息

宿主种属

Chicken

克隆

Monoclonal

克隆号

EPR20579

亚型

IgY

不含载体蛋白

No

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }

产品详情

This Chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab213511). By design, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Thiophilic Resin
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Abcam Product Promise

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详情请参阅我们的条款与条件。

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