重组Anti-mCherry抗体[EPR20579] - BSA and Azide free

• Flow Cyt

Lab

Flow Cytometry - Anti-mCherry antibody [EPR20579] - BSA and Azide free (AB232341)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213511). Flow cytometry analysis of 293T (human embryonic kidney epithelial cell) labeling mCherry with ab213511 at 1/500 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/5000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. Isotype control : Rabbit monoclonal IgG (ab172730) (Left).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [EPR20579] - BSA and Azide free (AB232341)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213511). Immunocytochemistry/ Immunofluorescence analysis of 293T (human embryonic kidney epithelial cell) labeling mCherry with ab213511 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. DAPI was used as nuclear counterstain. Confocal image showing positive staining in 293T cells transfected with a mCherry expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

• IP

Supplier Data

Immunoprecipitation - Anti-mCherry antibody [EPR20579] - BSA and Azide free (AB232341)

mCherry was immunoprecipitated from 0.35 mg of 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate with ab213511 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab213511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : 293T transfected with a His-tagged mCherry whole cell lysate 10 μg (Input).

Lane 2 : ab213511 IP in 293T transfected with a His-tagged mCherry whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213511 in 293T transfected with a His-tagged mCherry whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213511).

All lanes:

Immunoprecipitation - Anti-mCherry antibody [EPR20579] (<a href='/products/primary-antibodies/mcherry-antibody-epr20579-ab213511'>ab213511</a>)

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• I-ELISA

Lab

Indirect ELISA - Anti-mCherry antibody [EPR20579] - BSA and Azide free (AB232341)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213511). ELISA analysis of mPlum fluorescent protein,Osred fluorescent protein,tdTomato fluorescent protein,mcherry fluorescent protein at 1000 ng/mL with ab213511 at 1000-0 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.