Anti-mCherry抗体[1C51] (ab125096)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096)](https://www.abcam.cn/ps/products/125/ab125096/Images/ab125096-515294-anti-mcherry-antibody-1c51-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-mcherry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096)")
Key features and details
- Mouse monoclonal [1C51] to mCherry
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Species independent
- Isotype: IgG2a
概述
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产品名称
Anti-mCherry抗体[1C51]
参阅全部 mCherry 一抗 -
描述
小鼠单克隆抗体[1C51] to mCherry -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant full length protein corresponding to mCherry.
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阳性对照
- IHC-P: mCherry-transfected monkey brain tissue, Drosophila melanogaster eye imaginal discs and smooth muscle cells in rat bladder tissue. WB: HEK-293 cells transfected with mCherry-HA construct and mCherry recombinant protein. ICC/IF: mCherry-transfected HEK-293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.03% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
1C51 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125096于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/2000. Detects a band of approximately 28 kDa.
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| ICC/IF | (3) |
1/500.
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| IHC-P | (4) |
1/2000.
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| 说明 |
|---|
|
WB
1/2000. Detects a band of approximately 28 kDa. |
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ICC/IF
1/500. |
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IHC-P
1/2000. |
靶标
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相关性
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096)
Chromogenic immunostaining of formalin fixed paraffin embedded mCherry transfected monkey brain with mouse mAb to mCherry, dilution 1/2000, detected in DAB (brown) following the ImmPress method. Hematoxylin (blue) was used as the counterstain. ab125096 specifically detected the soma and axons of mCherry positive neurons in the cortex, as expected for this model.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096)](https://www.abcam.cn/ps/products/125/ab125096/Images/ab125096-318506-anti-mcherry-antibody-1c51-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096)Baumgartner R et al. The RNA-binding proteins FMR1, rasputin and caprin act together with the UBA protein lingerer to restrict tissue growth in Drosophila melanogaster. PLoS Genet 9:e1003598 (2013).4% paraformaldehyde fixed (at RT for 20 min and blocked with 2% NDS in 0.3% PBT) Drosophila melanogaster eye imaginal discs stained for mCherry (Red) using ab125096.
Analysis of rin hypomorphic alleles and the genomic rescue transgene GrinCherry. (A–C'') Negatively marked 72 h old rin2 (A, A''), (C, C'') mutant clones (induced with the FLP/FRT system) in eye imaginal discs of third instar larvae. Rin-Cherry levels expressed from the GrinCherry are autonomously increased in the rin mutant clones (A' and C'). The scale bar represents 50 µm.
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![Western blot - Anti-mCherry antibody [1C51] (ab125096)](https://www.abcam.cn/ps/products/125/ab125096/Images/ab125096-318532-anti-mcherry-antibody-1c51-western-blot.jpg)
Western blot - Anti-mCherry antibody [1C51] (ab125096)All lanes : Anti-mCherry antibody [1C51] (ab125096) at 1/1000 dilution
Lane 1 : Protein standard
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : HEK-293 cells transfected with mCherry-HA construct
Lane 4 : mCherry recombinant protein
Lane 5 : GFP recombinant protein
Lane 6 : HEK-293 transfected with GFP construct.Major band at about 30kDa corresponds to mCherry protein.
MCA-1C51 antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:5,000 in red which reveals band at 60kDa seen only in cell lysates.
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![Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [1C51] (ab125096)](https://www.abcam.cn/ps/products/125/ab125096/Images/ab125096-139267-anti-mcherry-antibody-1c51-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [1C51] (ab125096)mCherry-transfected HEK-293 (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling mCherry using ab125096 followed with a green secondary. DAPI was used to stain nuclei in blue. Cells are visualized using a confocal microscope. Transfected cells appear in yellow/orange colour.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096)](https://www.abcam.cn/ps/products/125/ab125096/Images/ab125096-262964-anti-mcherry-antibody-1c51-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096)Image courtesy of an anonymous Abreviewab125096 staining mCherry in smooth muscle cells in rat bladder by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.
Tissue was fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (186)
ab125096 被引用在 186 文献中.
- Xia B et al. Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2. Cell Discov 9:2 (2023). PubMed: 36609376
- Xiong X et al. Palmitoyl Transferase FonPAT2-Catalyzed Palmitoylation of the FonAP-2 Complex Is Essential for Growth, Development, Stress Response, and Virulence in Fusarium oxysporum f. sp. niveum. Microbiol Spectr 11:e0386122 (2023). PubMed: 36533963
- Onishi M et al. The GET pathway serves to activate Atg32-mediated mitophagy by ER targeting of the Ppg1-Far complex. Life Sci Alliance 6:N/A (2023). PubMed: 36697253
- Khalfi P et al. Antagonism of ALAS1 by the Measles Virus V protein contributes to degradation of the mitochondrial network and promotes interferon response. PLoS Pathog 19:e1011170 (2023). PubMed: 36802406
- Yokota S et al. Kif5a Regulates Mitochondrial Transport in Developing Retinal Ganglion Cells In Vitro. Invest Ophthalmol Vis Sci 64:4 (2023). PubMed: 36862119