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重组RabMAb

重组Anti-MCH抗体[EPR23778-94] - BSA and Azide free (ab274423)

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Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
  • Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
  • Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23778-94] to MCH - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-MCH抗体[EPR23778-94] - BSA and Azide free
    参阅全部 MCH 一抗

  • 描述

    兔单克隆抗体[EPR23778-94] to MCH - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    This antibody recognizes the PMCH chain.

  • 经测试应用

    适用于: IHC-Fr, IHC-Pmore details
    不适用于: ICC/IF,IP or WB

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human hypothalamus tissue; Mouse hypothalamus tissue; Rat hypothalamus tissue. IHC-Fr: Mouse hypothalamus tissue; Rat hypothalamus tissue.

  • 常规说明

    ab274423 is the carrier-free version of ab274415.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    pH: 7.2
    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR23778-94

  • 同种型

    IgG

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab274423于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for ICC/IF,IP or WB.

靶标

  • 功能

    MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.

  • 组织特异性

    Predominantly expressed in lateral hypothalamus, also detected in pallidum, neocortex and cerebellum. Also found in thymus, brown adipose tissue, duodenum and testis (spermatogonia, early spermatocytes and Sertoli cells). No expression in peripheral blood. In brain exclusively mature MCH and NEI peptides are present. In peripheral tissues a large product, encompassing the NEI and MCH domains of the precursor, is found predominantly.

  • 序列相似性

    Belongs to the melanin-concentrating hormone family.

  • 翻译后修饰

    Differentially processed in the brain and in peripheral organs producing two neuropeptides; NEI and MCH. A third peptide, NGE, may also be produced. Preferential processing in neurons by prohormone convertase 2 (PC2) generates NEI. MCH is generated in neurons of the lateral hypothalmic area by several prohormone convertases including PC1/3, PC2 and PC5/6.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • MCH antibody
      • MCH_HUMAN antibody
      • Melanin-concentrating hormone antibody
      • NEI antibody
      • Neuropeptide E-I antibody
      • Neuropeptide G-E antibody
      • NGE antibody
      • Pmch antibody
      see all

    图片

    • Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
      Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)

      This data was developed using ab274415, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hypothalamus tissue labeling PMCH with ab274415 at 1/500 (1.084 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)

      This data was developed using ab274415, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hypothalamus (PMID: 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
      Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)

      This data was developed using ab274415, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hypothalamus tissue labeling PMCH with ab274415 at 1/500 (1.084 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)

      This data was developed using ab274415, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hypothalamus (PMID: 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)

      This data was developed using ab274415, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human hypothalamus (PMID: 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)
      Anti-MCH antibody [EPR23778-94] - BSA and Azide free (ab274423)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab274423？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab274423 尚未被引用在任何文献中。

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