Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-MCEMP1抗体(ab121447)

Submit a review Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEMP1 antibody (ab121447)
  • Western blot - Anti-MCEMP1 antibody (ab121447)

Key features and details

  • Rabbit polyclonal to MCEMP1
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-MCEMP1抗体
    参阅全部 MCEMP1 一抗

  • 描述

    兔多克隆抗体to MCEMP1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment corresponding to amino acids 111-187 of Human MCEMP1 (Q8IX19).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • Human bone marrow; HEK293T cell MCEMP1 over-expression lysate

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab121447于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
说明
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB
Use a concentration of 0.04 - 0.4 µg/ml.

靶标

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEMP1 antibody (ab121447)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEMP1 antibody (ab121447)
    ab121447, at 1/150 dilution, staining MCEMP1 in paraffin-embedded Human bone marrow tissue by Immunohistochemistry, showing strong cytoplasmic positivity in bone marrow poietic cells.
  • Western blot - Anti-MCEMP1 antibody (ab121447)
    Western blot - Anti-MCEMP1 antibody (ab121447)
    All lanes : Anti-MCEMP1 antibody (ab121447) at 1/250 dilution

    Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
    Lane 2 : Negative control (vector only transfected HEK293T lysate)
    Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

文献 (1)

发表研究结果有使用 ab121447？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab121447 被引用在 1 文献中.

  • Chen JX  et al. Silence of long noncoding RNA NEAT1 exerts suppressive effects on immunity during sepsis by promoting microRNA-125-dependent MCEMP1 downregulation. IUBMB Life 71:956-968 (2019). PubMed: 30883005

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

Question

Publication IHC par Emoto.
Kits EpiSeeker
Anti-CEMP1 souris

Read More
Answer


1) La publication que j'ai mentionnée à propos du démasquage antigène en IHC est la suivante: Emoto et al., J Histochem Cytochem. 2005 53 (11) : 1311-21.
2) Les détails concernant les kits EpiSeeker sont disponibles via le lien suivant: www.abcam.com/episeeker.
3) Nous avons 2 anti-CEMP1, le ab134231 et le ab121447, tous 2 testés chez l'homme. Je n'ai malheureusement pas trouvé la séquence de la CEMP1 murine et je n'ai donc pas pu déterminer l'homologie de séquence entre les immunogènes et la protéine cible.
Si vous souhaitez tester un de ces 2 anticorps anti-CEMP1 chez la souris, je pourrai vous offrir une offre de test :
1. M'informer que vous souhaitez tester ab134231 ou ab121447 chez la souris et je vous enverrai un code promotionnel (valable 4 mois) à utiliser après obtention et envoi de vos résultats,
2. Acheter ab134231 ou ab121447,
3. Nous envoyer vos résultats (positifs ou négatifs) obtenus chez la souris sous forme d'Abreview (www.abcam.com/abreviews). Ceci activera le code.
4. Commander gratuitement un anticorps primaire Abcam de votre choix en utilisant le code promotionnel.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com