Anti-MCC抗体(ab125920)
Key features and details
- Rabbit polyclonal to MCC
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MCC抗体 -
描述
兔多克隆抗体to MCC -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 1-316 of Human MCC (P23508).
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阳性对照
- IMR32 and U-87 MG whole cell lysates; Human Colon carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125920于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 93 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 93 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation. -
组织特异性
Expressed in a variety of tissues. -
序列相似性
Belongs to the MCC family. -
细胞定位
Cell membrane. Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4163 Human
- Entrez Gene: 328949 Mouse
- Entrez Gene: 307449 Rat
- Omim: 159350 Human
- SwissProt: P23508 Human
- Unigene: 593171 Human
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别名
- Colorectal mutant cancer protein antibody
- CRCM_HUMAN antibody
- DKFZp762O1615 antibody
see all
图片
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Western blot - Anti-MCC antibody (ab125920)Anti-MCC antibody (ab125920) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCC antibody (ab125920)ab125920, at 1/250 dilution, staining MCC in Paraffin-embedded Human Colon carcinoma tissue by immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125920 尚未被引用在任何文献中。