Anti-MCAK抗体[2488C3a] (ab50778)
![Western blot - Anti-MCAK antibody [2488C3a] (ab50778)](https://www.abcam.cn/ps/products/50/ab50778/Images/ab50778-270786-ab50778KOWB.jpg "Western blot - Anti-MCAK antibody [2488C3a] (ab50778)")
Key features and details
- Mouse monoclonal [2488C3a] to MCAK
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-MCAK抗体[2488C3a]
参阅全部 MCAK 一抗 -
描述
小鼠单克隆抗体[2488C3a] to MCAK -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant human MCAK fragment
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. -
克隆
单克隆 -
克隆编号
2488C3a -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50778于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa. |
靶标
-
功能
Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. -
组织特异性
Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. -
序列相似性
Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
Contains 1 kinesin-motor domain. -
发展阶段
Isoform 2 is expressed in fetal testis. -
结构域
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. -
细胞定位
Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2. - Information by UniProt
-
数据库链接
- Entrez Gene: 11004 Human
- Omim: 604538 Human
- SwissProt: Q99661 Human
- Unigene: 720061 Human
-
别名
- 4930402F02Rik antibody
- CT139 antibody
- ESTM5 antibody
see all
图片
-
Western blot - Anti-MCAK antibody [2488C3a] (ab50778)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: MCAK knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab50778 observed at 90 kDa. Red - loading control, ab176560, observed at 50 kDa.ab50778 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. Ab50778 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-MCAK antibody [2488C3a] (ab50778)](https://www.abcam.cn/ps/products/50/ab50778/Images/BMR00446.jpg)
Western blot - Anti-MCAK antibody [2488C3a] (ab50778)Anti-MCAK antibody [2488C3a] (ab50778) + immunizing peptide
Predicted band size: 81 kDa
数据表及文件
-
Datasheet download
文献 (2)
ab50778 被引用在 2 文献中.
- D'Angelo L et al. MCAK-mediated regulation of endothelial cell microtubule dynamics is mechanosensitive to myosin-II contractility. Mol Biol Cell 28:1223-1237 (2017). PubMed: 28298485
- Braun A et al. Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells. J Cell Biol 206:97-112 (2014). PubMed: 25002679
