AB248980

重组Anti-MC4-R抗体[EPR6534(2)(ABC)] - BSA and Azide free

Anti-MC4-R antibody [EPR6534(2)(ABC)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MC4-R antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

查看别名

Melanocortin receptor 4, MC4-R, MC4R

1 Images

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Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] - BSA and Azide free (AB248980)

This data was developed using ab150419, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (<a href='/products/primary-antibodies/mc4-r-antibody-epr65342abc-ab150419'>ab150419</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW 264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Lane 8:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 36 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-MC4-R antibody [EPR6534(2)(ABC)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6534(2)(ABC)

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab248980 is the carrier-free version of ab150419.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The melanocortin-4 receptor also known as MC4-R is a G protein-coupled receptor found within the melanocortin receptor family. It has a molecular mass of approximately 38 kDa. MC4-R is expressed in the central nervous system with high levels in the hypothalamus a region that regulates energy balance and homeostasis. The receptor is activated by several peptide ligands including alpha-melanocyte-stimulating hormone (α-MSH) and it interacts with specific signaling cascades through G proteins.

Biological function summary

The actions through MC4-R are important for energy regulation and appetite control. As part of the melanocortin system MC4-R influences feeding behavior and energy expenditure. Its activation by endogenous or synthetic MC4-R activators suppresses appetite and increases energy consumption. The receptor does not work alone; it forms part of a signaling complex involving other receptor proteins such as MC3-R. The activity of MC4-R also connects to the regulation of body weight and influences fat mass.

Pathways

MC4-R plays an important role in the leptin-melanocortin pathway and the central regulation of energy homeostasis. Leptin a hormone produced by adipose tissues acts upstream by enhancing α-MSH production which in turn activates MC4-R. This activation leads to reduced food intake and increased thermogenesis. Moreover MC4-R interacts with another important signaling protein AGRP (Agouti-related peptide) which acts as an antagonist opposing the effects of α-MSH and regulating energy balance in a complex feedback system.

Mutations or dysregulation of MC4-R are linked to obesity and metabolic syndrome. About 5% of severe early-onset obesity cases involve mutations in the MC4-R gene. The receptor influences the risk for obesity-related conditions through its interactions with AGRP and α-MSH. Additionally compromised MC4-R signaling can lead to disorders such as cachexia where it impacts appetite and metabolism related to conditions like cancer or chronic illness. Defective MC4-R activity therefore is important in understanding the intricate balance between appetite energy expenditure and body mass control.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha- and beta-MSH) and corticotropin/ACTH, which are peptide products of the POMC precursor (PubMed : 12646665, PubMed : 14764818, PubMed : 25163632, PubMed : 32327598, PubMed : 33858992, PubMed : 8392067). Functions as a central component of the leptin-melanocortin pathway, which is essential for maintaining energy homeostasis (PubMed : 32327598, PubMed : 33858992). Upon activation, couples to G(s) protein, stimulating adenylate cyclase and the cAMP-dependent signaling pathway, which promotes anorexogenic signaling in the hypothalamus and contributes to a negative energy balance (PubMed : 12588803, PubMed : 14764818, PubMed : 25163632, PubMed : 33858992). Regulates food intake : activation by agonists suppresses appetite, whereas the antagonist Agouti-related protein/AGRP precludes agonist-induced signaling, thereby stimulating appetite (PubMed : 9311920). Modulates the firing activity of neurons in paraventricular nucleus (PVN) of the hypothalamus via alpha-MSH and AGRP regulation of inwardly rectifying potassium channel KCNJ13 closure, independently of G(s) signaling (PubMed : 32327598). In the PVN, also interacts with opsin 3/OPN3, which couples to G(i/o) proteins to inhibit MC4R-mediated cAMP signaling, thereby promoting food intake (PubMed : 39951488). In intestinal epithelial cells, contributes to inhibition of hepatic glucose production via nesfatin-1/NUCB2, leading to increased cAMP levels and glucagon-like peptide 1 (GLP-1) secretion (PubMed : 39562740). Interaction with MGRN1 displaces the G(s) protein, further decreasing MC4R signaling activity (PubMed : 19737927). Also activated by gamma-MSH, though with low potency (PubMed : 8392067).

See full target information MC4R

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Abcam Product Promise

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重组Anti-MC4-R抗体[EPR6534(2)(ABC)] - BSA and Azide free