AB150419

重组Anti-MC4-R抗体[EPR6534(2)(ABC)]

Anti-MC4-R antibody [EPR6534(2)(ABC)]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal MC4-R antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

查看别名

Melanocortin receptor 4, MC4-R, MC4R

1 Images

Unknown

Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (AB150419)

All lanes:

Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW 264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Lane 8:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 36 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-MC4-R antibody [EPR6534(2)(ABC)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6534(2)(ABC)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The melanocortin-4 receptor also known as MC4-R is a G protein-coupled receptor found within the melanocortin receptor family. It has a molecular mass of approximately 38 kDa. MC4-R is expressed in the central nervous system with high levels in the hypothalamus a region that regulates energy balance and homeostasis. The receptor is activated by several peptide ligands including alpha-melanocyte-stimulating hormone (α-MSH) and it interacts with specific signaling cascades through G proteins.

Biological function summary

The actions through MC4-R are important for energy regulation and appetite control. As part of the melanocortin system MC4-R influences feeding behavior and energy expenditure. Its activation by endogenous or synthetic MC4-R activators suppresses appetite and increases energy consumption. The receptor does not work alone; it forms part of a signaling complex involving other receptor proteins such as MC3-R. The activity of MC4-R also connects to the regulation of body weight and influences fat mass.

Pathways

MC4-R plays an important role in the leptin-melanocortin pathway and the central regulation of energy homeostasis. Leptin a hormone produced by adipose tissues acts upstream by enhancing α-MSH production which in turn activates MC4-R. This activation leads to reduced food intake and increased thermogenesis. Moreover MC4-R interacts with another important signaling protein AGRP (Agouti-related peptide) which acts as an antagonist opposing the effects of α-MSH and regulating energy balance in a complex feedback system.

Mutations or dysregulation of MC4-R are linked to obesity and metabolic syndrome. About 5% of severe early-onset obesity cases involve mutations in the MC4-R gene. The receptor influences the risk for obesity-related conditions through its interactions with AGRP and α-MSH. Additionally compromised MC4-R signaling can lead to disorders such as cachexia where it impacts appetite and metabolism related to conditions like cancer or chronic illness. Defective MC4-R activity therefore is important in understanding the intricate balance between appetite energy expenditure and body mass control.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha- and beta-MSH) and corticotropin/ACTH, which are peptide products of the POMC precursor (PubMed : 12646665, PubMed : 14764818, PubMed : 25163632, PubMed : 32327598, PubMed : 33858992, PubMed : 8392067). Functions as a central component of the leptin-melanocortin pathway, which is essential for maintaining energy homeostasis (PubMed : 32327598, PubMed : 33858992). Upon activation, couples to G(s) protein, stimulating adenylate cyclase and the cAMP-dependent signaling pathway, which promotes anorexogenic signaling in the hypothalamus and contributes to a negative energy balance (PubMed : 12588803, PubMed : 14764818, PubMed : 25163632, PubMed : 33858992). Regulates food intake : activation by agonists suppresses appetite, whereas the antagonist Agouti-related protein/AGRP precludes agonist-induced signaling, thereby stimulating appetite (PubMed : 9311920). Modulates the firing activity of neurons in paraventricular nucleus (PVN) of the hypothalamus via alpha-MSH and AGRP regulation of inwardly rectifying potassium channel KCNJ13 closure, independently of G(s) signaling (PubMed : 32327598). In the PVN, also interacts with opsin 3/OPN3, which couples to G(i/o) proteins to inhibit MC4R-mediated cAMP signaling, thereby promoting food intake (PubMed : 39951488). In intestinal epithelial cells, contributes to inhibition of hepatic glucose production via nesfatin-1/NUCB2, leading to increased cAMP levels and glucagon-like peptide 1 (GLP-1) secretion (PubMed : 39562740). Interaction with MGRN1 displaces the G(s) protein, further decreasing MC4R signaling activity (PubMed : 19737927). Also activated by gamma-MSH, though with low potency (PubMed : 8392067).

See full target information MC4R

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Progress in neurobiology 238:102632 PubMed38821345

2024

A molecularly defined orbitofrontal cortical neuron population controls compulsive-like behavior, but not inflexible choice or habit.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie T Yount,Silu Wang,Aylet T Allen,Lauren P Shapiro,Laura M Butkovich,Shannon L Gourley

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22920 PubMed37078546

2023

Glutamatergic melanocortin-4 receptor neurons regulate body weight.

Applications

Unspecified application

Species

Unspecified reactive species

Haodong Liu,Xiaojing Li,Penghui Li,Rihan Hai,Jiacheng Li,Qi Fan,Xing Wang,Yujie Chen,Xiaojuan Cao,Xiaoyu Zhang,Ruifeng Gao,Kun Wang,Chenguang Du

Frontiers in molecular biosciences 9:787885 PubMed35433838

2022

Proteomic Analysis of Serum Lysine Acetylation in Uyghur Patients With T2DM.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Yin,Gang Feng,Chun Huang,Weijuan Cai

Bioengineered 13:5598-5612 PubMed34787056

2021

Demethyltransferase FTO alpha-ketoglutarate dependent dioxygenase (FTO) regulates the proliferation, migration, invasion and tumor growth of prostate cancer by modulating the expression of melanocortin 4 receptor (MC4R).

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Li,Lin Cao

View all publications

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重组Anti-MC4-R抗体[EPR6534(2)(ABC)]