Anti-MC2-R抗体(ab77347)
Key features and details
- Goat polyclonal to MC2-R
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MC2-R抗体
参阅全部 MC2-R 一抗 -
描述
山羊多克隆抗体to MC2-R -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Sheep, Horse, Cow, Dog, Chimpanzee
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免疫原
Synthetic peptide corresponding to Human MC2-R aa 9-21 (N terminal). (NP_000520.1)
Sequence:ENINNTARNNSDC
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阳性对照
- Human skin lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab77347 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77347于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Receptor for ACTH. This receptor is mediated by G proteins (G(s)) which activate adenylate cyclase. -
组织特异性
Melanocytes and corticoadrenal tissue. -
疾病相关
Defects in MC2R are the cause of glucocorticoid deficiency type 1 (GCCD1) [MIM:202200]; also known as familial glucocorticoid deficiency type 1 (FGD1). GCCD1 is an autosomal recessive disorder due to congenital insensitivity or resistance to adrenocorticotropin (ACTH). It is characterized by progressive primary adrenal insufficiency, without mineralocorticoid deficiency. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4158 Human
- Omim: 607397 Human
- SwissProt: P34974 Cow
- SwissProt: Q01718 Human
- SwissProt: Q9TU77 Sheep
- Unigene: 248144 Human
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别名
- ACTH receptor antibody
- ACTH-R antibody
- ACTHR antibody
see all
图片
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Western blot - Anti-MC2-R antibody (ab77347)Anti-MC2-R antibody (ab77347) at 1 µg/ml + Human skin lysate in RIPA buffer at 35 µg
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (2)
ab77347 被引用在 2 文献中.
- Wierzbicka JM et al. Differentiation of Keratinocytes Modulates Skin HPA Analog. J Cell Physiol 232:154-66 (2017). PubMed: 27061711
- Wierzbicka JM et al. Bioactive forms of vitamin D selectively stimulate the skin analog of the hypothalamus-pituitary-adrenal axis in human epidermal keratinocytes. Mol Cell Endocrinol 437:312-322 (2016). PubMed: 27524410
