Anti-MBNL1 抗体

• WB

Project

Western blot - Anti-MBNL1 antibody (AB45899)

ab45899 recognises all three known isoforms to MBNL1

All lanes:

Western blot - Anti-MBNL1 antibody (ab45899) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat nuclear extract lysate (<a href='/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 42 kDa

Observed band size: 110 kDa,35 kDa,40 kDa,42 kDa,52 kDa

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• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBNL1 antibody (AB45899)

IHC image of ab45899 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45899, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody (AB45899)

ab45899 staining MBNL1 in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab45899 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• WB

CiteAb

Western blot - Anti-MBNL1 antibody (AB45899)

MBNL1 western blot using anti-MBNL1 antibody ab45899. Publication image and figure legend from García-Puga, M., Saenz-Antoñanzas, A., et al., 2020, Aging (Albany NY), PubMed 32310829.

ab45899 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab45899 please see the product overview.

DM1-derived fibroblasts present impaired metabolism. (A) Kinetic normalized OCR response in DM1 and control fibroblasts in basal conditions and after consecutive addition of Oligomycin 1.5 μM, FCCP 1.5 μM and Antimycin-A/Rotenone 1.5 μM. A representative experiment out of 3 is shown with 3 independent control cultures and 2 DM1. (B, C) Quantification of mitochondrial respiratory functions and coupling efficiency in DM1 (n=7) and control fibroblasts (n=3). (D) Representative energy map and (E) Quantification of metabolic potential of DM1 and control fibroblasts. Stressed indicates the values of OCR and ECAR after the injection of oligomycin and FCCP simultaneously. Results are obtained from controls (n=3) and DM1 (n=5) cultures. (F) Representative immunoblots of phospho-AKT, AKT, DMPK and MBNL1 in DM1-derived fibroblasts and healthy controls (n=3).

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