AB264217

Anti-MBD4/MED1抗体

Name: MBD4/MED1抗体 (ab264217)| Abcam中文官网
Brand: Abcam Limited
SKU: AB264217
Price: 4863 CNY
Availability: InStock

Anti-MBD4/MED1 antibody

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Rabbit Polyclonal MBD4/MED1 antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MBD4 aa 250-300.

查看别名

MED1, MBD4, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase

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Supplier Data

Immunoprecipitation - Anti-MBD4/MED1 antibody (AB264217)

MBD4/MED1 was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264217 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using an alternative MBD4/MED1 antibody at 0.1 μg/ml.

Lane 1 : ab264217 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-MBD4/MED1 antibody (ab264217)

Predicted band size: 66 kDa

true

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human MBD4 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

O95243

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MBD4 protein also known as MED1 is essential in DNA repair and gene regulation. MBD4 contains a methyl-CpG-binding domain allowing it to bind specifically to methylated DNA. The protein has a molecular weight of about 63 kDa. It is expressed in various tissues with higher levels in bone marrow and lymphoid tissues. MBD4 can recognize and excise mismatched T/G base pairs at methylated CpG sites which is important for maintaining genomic stability.

Biological function summary

MBD4 participates in the protection of genome integrity. It is involved in the DNA demethylation process ensuring proper gene expression patterns. MBD4 functions as part of complex DNA repair machinery. By working with other repair proteins MBD4 facilitates the correction of errors arising during DNA replication and demethylation reducing the risk of mutation accumulation and genomic instability.

Pathways

MBD4 plays a significant role in the base excision repair (BER) pathway. This pathway is key for correcting DNA damage caused by deamination and other forms of DNA alteration. In the BER pathway MBD4 cooperates with proteins like DNA glycosylases to identify and remove incorrect bases from the DNA strand. MBD4 also interacts with transcriptional regulation pathways influencing how genes are expressed in response to changes in the epigenetic landscape.

The malfunction of MBD4 links to colorectal cancer and other forms of cancer. Deficiency or mutations in the MBD4 gene increase the likelihood of mutation accumulation leading to tumorigenesis. MBD4's interactions with other proteins involved in DNA repair such as MLH1 highlight its role in maintaining DNA integrity. Genetic instability from impaired MBD4 function could trigger or exacerbate progression of cancerous conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G : T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G : U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

See full target information MBD4

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Anti-MBD4/MED1抗体

Anti-MBD4/MED1 antibody

Immunoprecipitation - Anti-MBD4/MED1 antibody (AB264217)