Anti-MBD2抗体(ab224827)
Key features and details
- Rabbit polyclonal to MBD2
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MBD2抗体
参阅全部 MBD2 一抗 -
描述
兔多克隆抗体to MBD2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human MBD2 aa 361-411. The exact sequence is proprietary. (NP_003918.1).
Database link: Q9UBB5 -
阳性对照
- IHC-P: Human prostate carcinoma tissue. WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224827于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-5 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 43 kDa.
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| 说明 |
|---|
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at 2-5 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 43 kDa. |
靶标
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功能
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. -
组织特异性
Highly expressed in brain, heart, kidney, stomach, testis and placenta. -
序列相似性
Contains 1 MBD (methyl-CpG-binding) domain. -
细胞定位
Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase. - Information by UniProt
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数据库链接
- Entrez Gene: 8932 Human
- Entrez Gene: 17191 Mouse
- Omim: 603547 Human
- SwissProt: Q9UBB5 Human
- SwissProt: Q9Z2E1 Mouse
- Unigene: 25674 Human
- Unigene: 322 Mouse
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别名
- Demethylase antibody
- DMTase antibody
- MBD 2 antibody
see all
图片
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Western blot - Anti-MBD2 antibody (ab224827)All lanes : Anti-MBD2 antibody (ab224827) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 antibody (ab224827)Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for MBD2 using ab224827 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
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Immunoprecipitation - Anti-MBD2 antibody (ab224827)MBD2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab224827 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224827 at 1 µg/ml.
Lane 1: ab224827 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab224827 被引用在 1 文献中.
- Day RN et al. Direct visualization by FRET-FLIM of a putative mechanosome complex involving Src, Pyk2 and MBD2 in living MLO-Y4 cells. PLoS One 16:e0261660 (2021). PubMed: 34941939