Anti-MBD2 + MBD3抗体[106B691] (ab45027)
Key features and details
- Mouse monoclonal [106B691] to MBD2 + MBD3
- Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-MBD2 + MBD3抗体[106B691] -
描述
小鼠单克隆抗体[106B691] to MBD2 + MBD3 -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cyt (Intra), ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Xenopus laevis, Zebrafish -
免疫原
Synthetic peptide corresponding to Human MBD2 + MBD3 aa 200-300. Synthetic peptide:
CKAFMVTDEDIRKQEE
, corresponding to amino acids 215-230 of Human MBD3
Database link: Q9UBB5 -
阳性对照
- HeLa cells (nuclear fraction). IHC-P: Human placenta tissue Flow Cyt (Intra): HeLa cells.
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常规说明
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. -
克隆
单克隆 -
克隆编号
106B691 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45027于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/300.
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 33, 45 kDa.
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说明 |
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IHC-P
1/300. |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 33, 45 kDa. |
靶标
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相关性
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 497269 Chicken
- Entrez Gene: 53615 Human
- Entrez Gene: 8932 Human
- Entrez Gene: 17191 Mouse
- Entrez Gene: 17192 Mouse
- Entrez Gene: 362834 Rat
- Entrez Gene: 680172 Rat
- Entrez Gene: 398135 Xenopus laevis
see all -
别名
- Demethylase antibody
- DMTase antibody
- MBD 2 antibody
see all
图片
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All lanes : Anti-MBD2 + MBD3 antibody [106B691] (ab45027) at 2 µg/ml
Lane 1 : HeLa cell lysate (nuclear fraction) without immunising peptide
Lane 2 : HeLa cell lysate (nuclear fraction) with immunising peptide
Predicted band size: 33, 45 kDa
Observed band size: 33,45 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 40 kDa. We are unsure as to the identity of these extra bands. -
Overlay histogram showing HeLa cells stained with ab45027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45027, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)Image courtesy of an anonymous Abreview.ab45027 at 2µg/ml staining MBD2 + MBD3 in human glioma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed and samples were blocked using 10% BSA. The secondary used was an HRP conjugated anti rabbit/mouse mix. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)Immunohistochemistry analysis of immersion fixed paraffin-embedded sections of human placenta labeling MBD2 + MBD3 with ab45027 at 1/300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab45027 被引用在 11 文献中.
- Liao R et al. BCL11B and the NuRD complex cooperatively guard T-cell fate and inhibit OPA1-mediated mitochondrial fusion in T cells. EMBO J 42:e113448 (2023). PubMed: 37737560
- Kraushaar DC et al. The gene repressor complex NuRD interacts with the histone variant H3.3 at promoters of active genes. Genome Res 28:1646-1655 (2018). PubMed: 30254051
- Xu M et al. MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells. Oncotarget 7:14476-85 (2016). WB . PubMed: 26910839
- Gómez-Del Arco P et al. The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis. Cell Metab 23:881-92 (2016). PubMed: 27166947
- Teperek M et al. Sperm and spermatids contain different proteins and bind distinct egg factors. Int J Mol Sci 15:16719-40 (2014). WB ; Frog . PubMed: 25244019