Anti-MAX抗体[73C5a] (ab53570)
Key features and details
- Mouse monoclonal [73C5a] to MAX
- Suitable for: ChIP, Flow Cyt, Dot blot, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
概述
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产品名称
Anti-MAX抗体[73C5a]
参阅全部 MAX 一抗 -
描述
小鼠单克隆抗体[73C5a] to MAX -
宿主
Mouse -
经测试应用
适用于: ChIP, Flow Cyt, Dot blot, WBmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant human MAX
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
ab53570 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
73C5a -
同种型
IgG2b -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53570于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ChIP |
Use at an assay dependent concentration. PubMed: 20797623
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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Dot blot |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa).
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说明 |
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ChIP
Use at an assay dependent concentration. PubMed: 20797623 |
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa). |
靶标
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功能
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. -
组织特异性
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. -
疾病相关
Pheochromocytoma -
序列相似性
Belongs to the MAX family.
Contains 1 bHLH (basic helix-loop-helix) domain. -
翻译后修饰
Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes. -
细胞定位
Nucleus. Cell projection, dendrite. - Information by UniProt
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数据库链接
- Entrez Gene: 4149 Human
- Omim: 154950 Human
- SwissProt: P61244 Human
- Unigene: 285354 Human
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别名
- bHLHd4 antibody
- bHLHd5 antibody
- bHLHd6 antibody
see all
图片
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Anti-MAX antibody [73C5a] (ab53570) + immunised recombinant protein
Predicted band size: 18 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
数据表及文件
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Datasheet download
文献 (13)
ab53570 被引用在 13 文献中.
- Sporbeck K et al. The ABL-MYC axis controls WIPI1-enhanced autophagy in lifespan extension. Commun Biol 6:872 (2023). PubMed: 37620393
- Sun YF et al. Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma. Nat Commun 12:4091 (2021). PubMed: 34215748
- Khazeem MM et al. Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta. FEBS Lett 594:3395-3405 (2020). PubMed: 32767399
- Lu W et al. The CARM1-p300-c-Myc-Max (CPCM) transcriptional complex regulates the expression of CUL4A/4B and affects the stability of CRL4 E3 ligases in colorectal cancer. Int J Biol Sci 16:1071-1085 (2020). PubMed: 32140074
- Mudersbach T et al. Epigenetic control of the angiotensin-converting enzyme in endothelial cells during inflammation. PLoS One 14:e0216218 (2019). PubMed: 31042763