Anti-Matriptase 2抗体- Catalytic domain (ab56182)
Key features and details
- Rabbit polyclonal to Matriptase 2 - Catalytic domain
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Matriptase 2抗体- Catalytic domain
参阅全部 Matriptase 2 一抗 -
描述
兔多克隆抗体to Matriptase 2 - Catalytic domain -
宿主
Rabbit -
特异性
ab56182 reacts with human Matripase 2 (TMPRSS6), and does not react with the other TMPRSSs. ab56182 recognizes all but the 2 truncated forms of human Matripase 2. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide based on the catalytic domain of Matriptase 2 (Human)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab56182于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
应用说明
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 89 kDa.
Note: Glycosylation and other posttranslational modifications make matriptase 2 run at higher than expected mass on reduced SDS PAGE gels.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Note: Glycosylation and other posttranslational modifications make matriptase 2 run at higher than expected mass on reduced SDS PAGE gels.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Required to sense iron deficiency. Overexpression suppresses activation of the HAMP promoter. -
组织特异性
Liver specific. -
疾病相关
Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA) [MIM:206200]; also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 3 LDL-receptor class A domains.
Contains 1 peptidase S1 domain. -
结构域
Cytoplasmic domain mediates HAMP suppression via proximal promoter element(s). -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 164656 Human
- Omim: 609862 Human
- SwissProt: Q8IU80 Human
- Unigene: 370885 Human
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别名
- IRIDA antibody
- Matriptase-2 antibody
- Membrane bound mosaic serine proteinase matriptase 2 antibody
see all
图片
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Western blot - Anti-Matriptase 2 antibody - Catalytic domain (ab56182)All lanes : Anti-Matriptase 2 antibody - Catalytic domain (ab56182) at 1/1000 dilution
All lanes : Cell media from human amnion-derived WISH cells
Predicted band size: 89 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab56182 被引用在 3 文献中.
- Krijt J et al. Matriptase-2 and Hemojuvelin in Hepcidin Regulation: In Vivo Immunoblot Studies in Mask Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33800732
- Gurieva I et al. Erythropoietin administration increases splenic erythroferrone protein content and liver TMPRSS6 protein content in rats. Blood Cells Mol Dis 64:1-7 (2017). WB ; Rat . PubMed: 28282554
- Frýdlová J et al. Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats. PLoS One 11:e0148540 (2016). WB . PubMed: 26845567