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Anti-Matrin 3抗体(ab70336)

Reviews (1)Q&A (4)References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (ab70336)
  • Immunoprecipitation - Anti-Matrin 3 antibody (ab70336)

Key features and details

  • Rabbit polyclonal to Matrin 3
  • Suitable for: IHC-P, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Matrin 3抗体
    参阅全部 Matrin 3 一抗

  • 描述

    兔多克隆抗体to Matrin 3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, IPmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus

  • 免疫原

    Synthetic peptide corresponding to a region between residues 475 and 500 of human Matrin 3 (NP_061322.2)

  • 阳性对照

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab70336于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP (1)
Use at 1-4 µg/mg of lysate.
说明
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at 1-4 µg/mg of lysate.

靶标

  • 功能

    May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs.

  • 疾病相关

    Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders.

  • 序列相似性

    Contains 1 matrin-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.

  • 细胞定位

    Nucleus matrix.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ALS21 antibody
    • KIAA0723 antibody
    • Matr3 antibody
    • MATR3_HUMAN antibody
    • Matrin-3 antibody
    • Matrin3 antibody
    • MPD2 antibody
    • VCPDM antibody
    • Vocal cord and pharyngeal weakness with distal myopathy antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (ab70336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (ab70336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Matrin 3 with ab70336 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-Matrin 3 antibody (ab70336)
    Immunoprecipitation - Anti-Matrin 3 antibody (ab70336)
    Detection of Human Matrin 3 by Western Blot of Immunprecipitate. Lane 1: HeLa whole cell lysates immunoprecipitated using ab70336 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 2: an irrelevant antibody was used for immunoprecipitation. All lanes: ab70336 at 3µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

文献 (4)

发表研究结果有使用 ab70336？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab70336 被引用在 4 文献中.

  • Boehringer A  et al. ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export. Sci Rep 7:14529 (2017). PubMed: 29109432
  • Li Y  et al. Immunoprecipitation and mass spectrometry defines an extensive RBM45 protein-protein interaction network. Brain Res 1647:79-93 (2016). PubMed: 26979993
  • Quintero-Rivera F  et al. MATR3 disruption in human and mouse associated with bicuspid aortic valve, aortic coarctation and patent ductus arteriosus. Hum Mol Genet 24:2375-89 (2015). WB . PubMed: 25574029
  • Kula A  et al. Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function. Retrovirology 8:60 (2011). IP ; Human . PubMed: 21771346

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Immunoprecipitation abreview for Anti-Matrin 3 antibody

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
10 µg
Specification
293T
Immuno-precipitation step
Protein A/G
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alessandro Marcello

Verified customer

提交于 Sep 30 2011

Question

Dear xxxxx,

This is to inform you that I have received the product. I will let you know how the experiments go.

Until then, thank you.

Read More
Answer

Thank you very much for letting me know.

I wish you all the best with your experiments and look forward to hearing how you get on.

Read More

Question

Dear Karin,

My telephone is: xxxx
Many greetings.


xxxxx

Read More
Answer

Thank you for confirming those details. I have now set up a new delivery for you to the address which you specified. This should be with you tomorrow, or at the latest Friday. The order number is xxxx (PO number FOCR xxxxx).

You should get confirmation of the Fedex tracking number once the shipment has been sent.

I look forward to hearing how you get on with your experiments. Until then I wish you all the best.

Read More

Question

Dear xxxx,

I promise to send you the results of both antibodies (in terms of positive and negative articulation). But, it would have been nice if you send to me the other antibody now because this antibody is indeed for WB, though it is only predicted for mouse. I would have liked to do the experiments in the same day with the same sample.

Read More
Answer

Thank you for getting back in contact.

I can understand why you would like to test both antibodies at the same time, and therefore I am willing to offer you this as a goodwill gesture. In order to do this I will need the original order number for ab70336. If you do not have this to hand, a delivery address and an approximate date of delivery should be enough. If you can get this information to me before 4 pm (Netherlands time) I will be able to set up the shipment to be with you tomorrow.

I would appreciate it though, if you do have trouble with the western blotting (particularly ab70336) if you could let me know prior to publishing the Abreview as there may be suggestions I can make in optimising the protocol.

I look forward to receiving your response.

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Question

Dear xxxx,

I promised you to send an e-mail to you over the above mentioned product. As discussed, I thought that this antibody can be used for WB. It appears not. First, can you please tell me why it is not suitable for WB? Second, what solution can you provide me? The only other anti-matrin 3 of Abcam that may be used for WB is the product with the catalog No. Ab51081, even here there seems uncertainy because I want to use the anti-body against mouse. Even for the latter antibody, you indicate that it can be used for human, and that it is predicted for mouse and some other species. Nevertheless, at least it is predicted for mouse. The referenc that I told you yesterday: "Kula et al. Characterization of the HIV-1 RNA associated proteome identifies matrin 3 as a nuclear cofactor of Rev function" published in Retrovirology (2011) 8:60. On close examination, I noticed these authors used Ab70336 for immunoprecipitation and for the WB it seems they have used another antibody from VIVA.

I am waiting for your reaction.

Yours sincerely,

Read More
Answer

Thank you for contacting us and I'm sorry in the delay in getting back to you.

As discussed over the phone yesterday, I am seeking more information from the source of this antibody to ascertain exactly why ab70336 was not deemed suitable for use in Western blotting. Once I have more information in this regard I will let you know.

Ab70336 has been used to detect both the mouse and human protein. This is the only anti-Matrin 3 antibody from our catalogue which has been used to detect the mouse protein. https://www.abcam.com/Matrin-3-antibody-ab51081.html, which has been validated in western blotting is however predicted to react with the mouse protein due to the sequence homology between the immunogen used to raise the antibody and the mouseMatrin 3 protein. These sequences share 98% homology (please refer to the attachment of this email). As mouse samples have not been tested with this antibody, I cannot however guarantee that it will.

I think I would suggest it would be worth trying ab70336 with your mouse samples in western blotting. If you find that it does not work then I can offer you ab51081 free of charge to try, if you will submit an Abreview for the two antibodies.This will only take 5 minutes to submit and would be invaluable data to our other customers looking for similar Matrin 3 antibodies as yourself.

I hope this information has been of help. I look forward to hearing how you would like to proceed.

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