重组Anti-Mast Cell Tryptase抗体[EPR9522] - BSA and Azide free (ab271916)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)](https://www.abcam.cn/ps/products/271/ab271916/Images/ab271916-1-ab151757158877antimastcelltryptaseantibodyepr9522immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9522] to Mast Cell Tryptase - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Mast Cell Tryptase抗体[EPR9522] - BSA and Azide free
参阅全部 Mast Cell Tryptase 一抗 -
描述
兔单克隆抗体[EPR9522] to Mast Cell Tryptase - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human skin, colon cancer or tonsil lysate, Human colon tissue.
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常规说明
ab271916 is the carrier-free version of ab151757.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9522 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271916于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| IHC-P | (2) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
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| 说明 |
|---|
|
IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa. |
靶标
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相关性
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation. -
细胞定位
Secreted. Note: released from the secretory granules upon mast cell activation. -
数据库链接
- Entrez Gene: 7177 Human
- Entrez Gene: 100503895 Mouse
- Entrez Gene: 54271 Rat
- Omim: 191080 Human
- SwissProt: Q15661 Human
- SwissProt: Q02844 Mouse
- SwissProt: P27435 Rat
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别名
- alpha II antibody
- Lung tryptase antibody
- Mast cell alpha II tryptase antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)
Immunohistochemical analysis of paraffin embedded Human tissue labeling Mast Cell Tryptase with ab151757 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151757). -
![Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)](https://www.abcam.cn/ps/products/271/ab271916/Images/ab271916-2-benefits-of-recombinant-antibodies.png)
Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271916 尚未被引用在任何文献中。
