AB134932

重组Anti-Mast Cell Tryptase抗体[EPR8476]

Name: Mast Cell Tryptase抗体[EPR8476] (ab134932)| Abcam中文官网
Brand: Abcam Limited
SKU: AB134932
Price: 3004 CNY
Availability: InStock
Rating: 4 (5 reviews)

Anti-Mast Cell Tryptase antibody [EPR8476]

RabMAb
Recombinant
了解详情

(5 Reviews)

(28 Publications)

Rabbit Recombinant Monoclonal TRYB1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 28 publications.

查看别名

TPS1, TPS2, TPSB1, TPSAB1, Tryptase alpha/beta-1, Tryptase-1, Tryptase I, Tryptase alpha-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (AB134932)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (AB134932)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling Mast Cell Tryptase with Purified ab134932 at 1 : 5000 dilution (0.65 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (AB134932)

Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling Mast Cell Tryptase using ab134932 at a 1/500 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (AB134932)

Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human tonsil tissue, labelling Mast Cell Tryptase using ab134932 at a 1/500 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

AbReview35546****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] (AB134932)

ab14404 staining Mast Cell Tryptase in Mouse small intestine tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Cells were fixed with paraformaldehyde, blocked with 5% serum for 60 minutes at 21°C and heat mediated antigen retrieval was performed in 10mM Citrate buffer pH6.0. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor^®488-conjugated Donkey anti-rabbit Ipolyclonal(1/400) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

不同偶联物与剂型 (7)

Carrier free

Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Mast Cell Tryptase antibody [EPR8476]
578 PE

PE Anti-Mast Cell Tryptase antibody [EPR8476]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Mast Cell Tryptase antibody [EPR8476]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Mast Cell Tryptase antibody [EPR8476]
660 APC

APC Anti-Mast Cell Tryptase antibody [EPR8476]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Mast Cell Tryptase antibody [EPR8476]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8476

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Lab re-tests in mouse and rat species were negative. Therefore, we do not guarantee this antibody in these species.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.For unpurified use at 1/500 - 1/1000." } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed : 18854315).

See full target information TPSAB1

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文献 (28)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112988 PubMed41054545

2025

Multiregional single-cell transcriptomics reveals an association between partial EMT and immunosuppressive states in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Seunghoon Kim,Hyun Jung Kee,Dahee Kim,Jinho Jang,Hyoung-Oh Jeong,Nam Suk Sim,Mischa Selig,Jana Ihlow,Livius Penter,Taejoo Hwang,David Whee-Young Choi,Kyoung Jun Lee,Jaewoong Lee,Young Min Park,Semin Lee,Yoon Woo Koh

Cells 12: PubMed37681909

2023

Human Mast Cells Upregulate Cathepsin B, a Novel Marker of Itch in Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Peter W West,Chiara Tontini,Haris Atmoko,Orsolya Kiss,Terence Garner,Rajia Bahri,Richard B Warren,Christopher E M Griffiths,Adam Stevens,Silvia Bulfone-Paus

Scientific reports 12:17851 PubMed36284123

2022

An efficient rAAV vector for protein expression in cortical parvalbumin expressing interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Tkatch,Kristina Rysevaite-Kyguoliene,Ignas Sabeckis,Deimante Sabeckiene,Dainius H Pauza,Gytis Baranauskas

JOR spine 5:e1229 PubMed36601380

2022

Fibrotic and inflammatory characteristics of epidural fat adjacent to the ossification area in patients with ossification of the ligament flavum.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Dou,Tianli Mao,Yunlong Ma,Fei Jia,Yu Liu,Xiaoguang Liu

Endocrinology 163: PubMed36156081

2022

Foxe1 Deletion in the Adult Mouse Is Associated With Increased Thyroidal Mast Cells and Hypothyroidism.

Applications

Unspecified application

Species

Unspecified reactive species

Grace Lim,Alexander Widiapradja,Scott P Levick,Kelly J McKelvey,Xiao-Hui Liao,Samuel Refetoff,Martyn Bullock,Roderick J Clifton-Bligh

Journal of inflammation research 14:2979-2991 PubMed34262323

2021

Overexpression of Neutrophil MMP-9 and HIF-1α May Contribute to the Finger-Like Projections Formation and Histo-Pathogenesis in Nasal Inverted Papilloma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Kai Sen Tan,Yan Yi Tu,Li Zhao,Jing Liu,Hsiao Hui Ong,De Yun Wang,Li Shi

Frontiers in immunology 12:622001 PubMed33737929

2021

Repurposing of the Antiepileptic Drug Levetiracetam to Restrain Neuroendocrine Prostate Cancer and Inhibit Mast Cell Support to Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Sulsenti,Barbara Frossi,Lucia Bongiovanni,Valeria Cancila,Paola Ostano,Irene Fischetti,Claudia Enriquez,Francesca Guana,Giovanna Chiorino,Claudio Tripodo,Carlo E Pucillo,Mario P Colombo,Elena Jachetti

Aging 12:21809-21836 PubMed33177244

2020

CCL5-dependent mast cell infiltration into the tumor microenvironment in clear cell renal cell carcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Tianjie Liu,Qing Xia,Haibao Zhang,Zixi Wang,Wenjie Yang,Xiaoyun Gu,Tao Hou,Yule Chen,Xinqi Pei,Guodong Zhu,Dalin He,Lei Li,Shan Xu

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 24:31-44 PubMed32488650

2020

Interleukin-17A derived from mast cells contributes to fibrosis in gastric cancer with peritoneal dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuya Gunjigake,Jun Kinoshita,Takahisa Yamaguchi,Hiroto Saito,Daisuke Fujimori,Toshihide Horiike,Shinichi Harada,Hidehiro Tajima,Itasu Ninomiya,Tetsuo Ohta,Sachio Fushida

Acta dermato-venereologica 100:adv00099 PubMed32176307

2020

The OX40 Axis is Associated with Both Systemic and Local Involvement in Atopic Dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Sophie Elsner,Michael Carlsson,Julie Kristine Stougaard,Uffe Nygaard,Matthias Buchner,Regina Fölster-Holst,Malene Hvid,Christian Vestergaard,Mette Deleuran,Bent Deleuran

View all publications

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重组Anti-Mast Cell Tryptase抗体[EPR8476]