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AB244804

重组Anti-MASPIN抗体[EPR20155-32] - BSA and Azide free (Capture)

Anti-MASPIN antibody [EPR20155-32] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal MASPIN antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

查看别名

PI5, SERPINB5, Serpin B5, Maspin, Peptidase inhibitor 5, PI-5

1 Images
Sandwich ELISA - Anti-MASPIN antibody [EPR20155-32] - BSA and Azide free (Capture) (AB244804)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-MASPIN antibody [EPR20155-32] - BSA and Azide free (Capture) (AB244804)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab238270).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20155-32

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab244804 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse MASPIN. The recommended pair for sandwich ELISA is:
Capture: ab244804, Mouse MASPIN Capture Antibody (unconjugated)
Detector: ab245077, Mouse MASPIN Detector Antibody (unconjugated)
The reference range value is 46.88 - 3000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MASPIN also known as SERPINB5 is a non-inhibitory serine protease inhibitor with a mass of approximately 42 kDa. It functions mechanically to regulate cell adhesion migration apoptosis and angiogenesis. MASPIN localizes mainly in the cytoplasm and nucleus and expresses predominantly in epithelial cells including those in the mammary gland prostate and keratinocytes in the skin. It acts as a tumor suppressor and researchers often use immunohistochemistry (IHC) to study its expression patterns in various tissues.
Biological function summary

MASPIN influences apoptosis and cell motility playing a critical role in tumor suppression. It does not form part of a complex but interacts directly with extracellular matrix components. MASPIN can inhibit angiogenesis by reducing the proliferation and migration of endothelial cells. Scientists have noted its role in maintaining normal cell architecture and inhibiting tumor cell invasion.

Pathways

Researchers place MASPIN in important signaling networks impacting cancer progression and metastasis. It participates in the PI3K/AKT pathway thereby influencing cell growth and survival. MASPIN also intersects with TGF-β signaling and related proteins like SMAD which mediate growth arrest and apoptosis. By altering these pathways MASPIN helps modulate responses to cellular stress and inhibit lethal growth in cancer cells.

MASPIN often associates with breast and prostate cancers. Its expression inversely correlates with tumor progression suggesting a protective role. MASPIN's interaction with proteins like p53 highlights its contribution to tumor suppression. Abnormal MASPIN presence links to aggressive disease phenotypes making it a potential biomarker for prognostic evaluation in oncology.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
See full target information SERPINB5
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搭配使用产品

Assay Kits

AB218269

Human MASPIN ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MASPIN ELISA Kit (AB218269)

  • 0
  • 0
Primary Antibodies

AB259618

Anti-MMP12 antibody [EPR22132-32] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-MMP12 antibody [EPR22132-32] - BSA and Azide free (Detector) (AB259618)

  • 0
  • 0
Primary Antibodies

AB244927

Anti-MCP2 antibody [EPR20081-86] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-MCP2 antibody [EPR20081-86] - BSA and Azide free (Detector) (AB244927)

  • 0
  • 0
Primary Antibodies

AB245083

Anti-MCP4 antibody [EPR19877-83] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-MCP4 antibody [EPR19877-83] - BSA and Azide free (Detector) (AB245083)

  • 0
  • 0
Primary Antibodies

AB32454

Anti-MAP2 antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

  • 5
  • 35

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