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重组RabMAb

重组Anti-MASP1抗体[EPR22804-121] - BSA and Azide free (Capture) (ab281184)

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Sandwich ELISA - Anti-MASP1 antibody [EPR22804-121] - BSA and Azide free (Capture) (ab281184)
  • Anti-MASP1 antibody [EPR22804-121] - BSA and Azide free (Capture) (ab281184)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22804-121] to MASP1 - BSA and Azide free (Capture)
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

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Human MASP1 Antibody Pair - BSA and Azide free (ab253624)

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概述

  • 产品名称

    Anti-MASP1抗体[EPR22804-121] - BSA and Azide free (Capture)
    参阅全部 MASP1 一抗
  • 描述

    兔单克隆抗体[EPR22804-121] to MASP1 - BSA and Azide free (Capture)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: Sandwich ELISAmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    ab281184 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MASP1. The recommended pair for sandwich ELISA is:
         Capture: ab281184, Human MASP1 Capture Antibody (unconjugated)
         Detector: ab281034, Human MASP1 Detector Antibody (unconjugated)
    The reference range value is 31.25-2000 pg/mL.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Constituent: 100% PBS
  • 无载体

    是
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR22804-121
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Complement
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
    • Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
  • Conjugation kits

    • Alkaline phosphatase Conjugation Kit - Lightning-Link® (ab102850)
    • HRP Conjugation Kit - Lightning-Link® (ab102890)
    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • sELISA pair antibody

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
    • Anti-MASP1 antibody [EPR22804-162] - BSA and Azide free (Detector) (ab281034)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab281184于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR22804-162] to MASP1 - BSA and Azide free (Detector) (ab281034).
说明
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR22804-162] to MASP1 - BSA and Azide free (Detector) (ab281034).

靶标

  • 相关性

    MASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown.
  • 细胞定位

    Secreted
  • 数据库链接

    • Entrez Gene: 5648 Human
    • Omim: 600521 Human
    • SwissProt: P48740 Human
    • 别名

      • Complement factor MASP-3 antibody
      • Complement factor MASP3 antibody
      • Complement-activating component of Ra-reactive factor antibody
      • CRARF antibody
      • Mannose binding lectin associated serine protease 1 antibody
      • Mannose binding protein associated serine protease antibody
      • Mannose-binding lectin-associated serine protease 1 antibody
      • Mannose-binding lectin-associated serine protease-1 antibody
      • MASP antibody
      • PRSS 5 antibody
      • Ra-reactive factor serine protease p100 antibody
      • RaRF antibody
      • Serine protease 5 antibody
      see all

    图片

    • Sandwich ELISA - Anti-MASP1 antibody [EPR22804-121] - BSA and Azide free (Capture) (ab281184)
      Sandwich ELISA - Anti-MASP1 antibody [EPR22804-121] - BSA and Azide free (Capture) (ab281184)
      Sandwich ELISA of ab253624 with the capture antibody ab281184 dilution at 2 µg/mL and detector antibody ab281034 dilution at 0.5 µg/mL.
    • Anti-MASP1 antibody [EPR22804-121] - BSA and Azide free (Capture) (ab281184)
      Anti-MASP1 antibody [EPR22804-121] - BSA and Azide free (Capture) (ab281184)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab281184?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab281184 尚未被引用在任何文献中。

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