Anti-MARS2抗体(ab130426)
Key features and details
- Rabbit polyclonal to MARS2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-MARS2抗体 -
描述
兔多克隆抗体to MARS2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment: WLGTVLHVAL ECLRVFGTLL QPVTPSLADK LLSRLGVSAS ERSLGELYFL PRFYGHPCPF EGRRLGPETG LLFPRLDQSR TWLVKAHRT, corresponding to amino acids 505-593 of Human MARS2 (UniProt: Q96GW9).
-
阳性对照
- RT4 cell lysates; Human stomach tissue.
-
常规说明
ab130426 is a mono-specific antibody.
The antibody solution should be gently mixed before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab130426于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
WB |
1/250 - 1/500. Predicted molecular weight: 67 kDa.
|
|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
1/250 - 1/500. Predicted molecular weight: 67 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
序列相似性
Belongs to the class-I aminoacyl-tRNA synthetase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
-
数据库链接
- Entrez Gene: 92935 Human
- Omim: 609728 Human
- SwissProt: Q96GW9 Human
- Unigene: 116602 Human
-
别名
- mars2 antibody
- Methionine tRNA ligase 2 antibody
- Methionine tRNA ligase 2 mitochondrial antibody
see all
图片
-
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments) and aggresome. Recommended concentration of ab130426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
All lanes : Anti-MARS2 antibody (ab130426) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 67 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARS2 antibody (ab130426)ab130426, at a dilution of 1/200, staining MARS2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab130426 被引用在 1 文献中.
- Gu J & Chu K Increased Mars2 expression upon microRNA-4661-5p-mediated KDM5D downregulation is correlated with malignant degree of gastric cancer cells. Cell Biol Int 45:2118-2128 (2021). PubMed: 34273914