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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MARK

Anti-MARK4抗体(ab5262)

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Submit a review Q&A (2)References (5)

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Western blot - Anti-MARK4 antibody (ab5262)
  • Western blot - Anti-MARK4 antibody (ab5262)

Key features and details

  • Goat polyclonal to MARK4
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-MARK4抗体
    参阅全部 MARK4 一抗
  • 描述

    山羊多克隆抗体to MARK4
  • 宿主

    Goat
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    IKRKPPPGCSDSPGV

    , corresponding to C terminal amino acids 674-688 of Human MARK4.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Human cerebellum tissue, mouse brain tissue, and U251 cell lysates.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MARK

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant human MARK4 protein (ab105211)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5262于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 75 kDa.

1 hour primary incubation is recommended for this product.

说明
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 75 kDa.

1 hour primary incubation is recommended for this product.

靶标

  • 功能

    Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).
  • 组织特异性

    Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).
  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • 发展阶段

    Expressed at all stages of the mitotic cell cycle.
  • 翻译后修饰

    Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
    Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).
  • 细胞定位

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.
  • Target information above from: UniProt accession Q96L34 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 57787 Human
    • Entrez Gene: 232944 Mouse
    • Omim: 606495 Human
    • SwissProt: Q96L34 Human
    • SwissProt: Q8CIP4 Mouse
    • Unigene: 34314 Human
    • Unigene: 260504 Mouse
    • 别名

      • FLJ90097 antibody
      • KIAA1860 antibody
      • MAP / microtubule affinity regulating kinase 4 antibody
      • MAP / microtubule affinity regulating kinase 4L antibody
      • MAP/microtubule affinity regulating kinase 4 antibody
      • MAP/microtubule affinity regulating kinase like 1 antibody
      • MAP/microtubule affinity-regulating kinase 4 antibody
      • MAP/microtubule affinity-regulating kinase-like 1 antibody
      • MARK 4 antibody
      • MARK 4L antibody
      • MARK L1 antibody
      • MARK4 antibody
      • MARK4 serine / threonine protein kinase antibody
      • MARK4 serine/threonine protein kinase antibody
      • MARK4_HUMAN antibody
      • MARK4S antibody
      • MARKL 1 antibody
      • MARKL1 antibody
      • Nbla00650 antibody
      • Putative protein product of Nbla00650 antibody
      see all

    图片

    • Western blot - Anti-MARK4 antibody (ab5262)
      Western blot - Anti-MARK4 antibody (ab5262)
      All lanes : Anti-MARK4 antibody (ab5262) at 0.3 µg/ml

      Lane 1 : Human cerebellum tissue lysate (35µg protein in RIPA buffer)
      Lane 2 : Human cerebellum tissue lysate (35µg protein in RIPA buffer) with Immunizing peptide
      Lane 3 : Mouse brain tissue lysate (35µg protein in RIPA buffer)
      Lane 4 : Mouse brain tissue lysate (35µg protein in RIPA buffer) with Immunizing peptide

      Developed using the ECL technique.

      Predicted band size: 75 kDa

    • Western blot - Anti-MARK4 antibody (ab5262)
      Western blot - Anti-MARK4 antibody (ab5262)
      Anti-MARK4 antibody (ab5262) at 1 µg/ml + U251 cell lysate (35µg protein in RIPA buffer)

      Developed using the ECL technique.

      Predicted band size: 75 kDa

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (5)

    发表研究结果有使用 ab5262?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5262 被引用在 5 文献中.

    • Li L  et al. Long non-coding RNA DLGAP1-AS1 promotes the progression of gastric cancer via miR-515-5p/MARK4 axis. Braz J Med Biol Res 54:e10062 (2021). PubMed: 34037089
    • Ortega MA  et al. Chronic venous disease patients show increased IRS-4 expression in the great saphenous vein wall. J Int Med Res 49:3000605211041275 (2021). PubMed: 34590920
    • Chen Y  et al. Capn4 regulates migration and invasion of ovarian carcinoma cells via targeting osteopontin-mediated PI3K/AKT signaling pathway. Oncol Lett 17:564-570 (2019). PubMed: 30655802
    • Wang L  et al. Prazosin protects myocardial cells against anoxia-reoxygenation injury via the extracellular signal-regulated kinase signaling pathway. Mol Med Rep 17:2145-2152 (2018). PubMed: 29207167
    • Magnani I  et al. Differential signature of the centrosomal MARK4 isoforms in glioma. Anal Cell Pathol (Amst) 34:319-38 (2011). PubMed: 22156016

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    I have a few questions regarding the product: What region was it made against? Which epitope was it made against? In which cell lines(s) does the protein express that could be used as a positive control? Does the antibody recognize a single band versus multiple bands? Thank you

    Read More

    Abcam community

    Verified customer

    Asked on May 25 2005

    Answer

    Thank you for your enquiry. The immunogen used was the synthetic peptide IKRKPPPGCSDSPGV, corresponding to C terminal amino acids 674-688 of Human MARK4. Please note that ab5262 is a "Fast-Track" antibody (like your boot camp antibodies!). Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. We realise that these products might be very suitable for your research and do not wish to delay this; therefore, we release them early with the following understanding: As Fast-Track antibodies are not yet fully characterised we are unable to offer our normal level of technical support. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. Preliminary Western blot testing with ab5262 showed the following: no signal obtained yet but low background observed in Human Brain and 293 lysates at up to 2µg/ml. We would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award 1,000 Abcam points to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. If you have any additional questions, just let us know.

    Read More

    Abcam Scientific Support

    回复于 May 25 2005

    Question

    I am interested in your MARK4 antibody. The datasheet states it has been tested in "peptide ELISA." I would like more specific information about the ELISA format (primary, secondary antibodies, procedure, etc.). Also, I am interested in antibodies for MARK 1,2,and 3 and for tau. If you have any of these planned for fast-track availability I am very interested in testing them. Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 19 2005

    Answer

    Thank you for your enquiry and your interest in our products. The only ELISA we have done tests binding of the antibody to the peptide used for immunization. As the datasheet says our antibody detection limit dilution was found to be 1:32000. Important parts of the protocol: - Plates coated with immunizing peptide at 1ug/ml, 4C overnight - Plates blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05% Tween-20) for 1 hr at room temperature - Secondary antibody, Anti-goat Alkaline Phoshatase - Readout using p-nitrophenyl phosphate in diethanolamine buffer. Re MARK 1, 2, 3 currently we aren't making them currently. However, there are at least 10 antibodies against Tau (ab4841, ab4749, ab4846, ab4856, ab4858). We hope this will help!

    Read More

    Abcam Scientific Support

    回复于 Jan 24 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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