Anti-MAPK6/ERK3 (phospho S189)抗体(ab74032)
Key features and details
- Rabbit polyclonal to MAPK6/ERK3 (phospho S189)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MAPK6/ERK3 (phospho S189)抗体
参阅全部 MAPK6/ERK3 一抗 -
描述
兔多克隆抗体to MAPK6/ERK3 (phospho S189) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human MAPK6/ERK3 (phospho S189).
Sequence:H-L-Sp-E-G
Database link: Q16659 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab74032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74032于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
|
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| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). |
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IHC-P
1/50 - 1/100. |
靶标
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功能
Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle. -
组织特异性
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme. - Information by UniProt
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数据库链接
- Entrez Gene: 5597 Human
- Entrez Gene: 50772 Mouse
- Entrez Gene: 58840 Rat
- Omim: 602904 Human
- SwissProt: Q16659 Human
- SwissProt: Q61532 Mouse
- SwissProt: P27704 Rat
- Unigene: 411847 Human
see all -
别名
- ERK-3 antibody
- ERK3 antibody
- Extracellular signal regulated kinase 3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab74032, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising phosphopeptide.
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Western blot - Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032)All lanes : Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032) at 1/500 dilution
Lane 1 : Extracts from mouse brain
Lane 2 : Extracts from mouse brain with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab74032 被引用在 7 文献中.
- Brandolini L et al. Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions. Cell Death Dis 13:500 (2022). PubMed: 35614037
- Guo YP et al. Suppression of lncRNA HOTAIR alleviates RCC angiogenesis through regulating miR-126/EGFL7 axis. Am J Physiol Cell Physiol 320:C880-C891 (2021). PubMed: 33502949
- Li G et al. Exosomes-carried microRNA-26b-5p regulates microglia M1 polarization after cerebral ischemia/reperfusion. Cell Cycle 19:1022-1035 (2020). PubMed: 32208888
- Jia T et al. MiR-148a inhibits oral squamous cell carcinoma progression through ERK/MAPK pathway via targeting IGF-IR. Biosci Rep 40:N/A (2020). PubMed: 32202300
- He C et al. Runt-related transcription factor 1 contributes to lung cancer development by binding to tartrate-resistant acid phosphatase 5. Cell Cycle 18:3404-3419 (2019). PubMed: 31650885
- Liu YS & Wei B Over-expression of Bcl2-associated athanogene 2 in oral cancer promotes cellular proliferation and is associated with poor prognosis. Arch Oral Biol 102:164-170 (2019). PubMed: 31055249
- Ling S et al. Metformin potentiates the effect of arsenic trioxide suppressing intrahepatic cholangiocarcinoma: roles of p38 MAPK, ERK3, and mTORC1. J Hematol Oncol 10:59 (2017). PubMed: 28241849