重组Anti-MAPK6/ERK3抗体[EP1720Y] (ab53277)
![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.cn/ps/products/53/ab53277/Images/ab53277-395392-anti-mapk6erk-3-antibody-ep1720y-western-blot-human-knockout-lysate.jpg "Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1720Y] to MAPK6/ERK3
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MAPK6/ERK3抗体[EP1720Y]
参阅全部 MAPK6/ERK3 一抗 -
描述
兔单克隆抗体[EP1720Y] to MAPK6/ERK3 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MAPK6/ERK3 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q16659 -
阳性对照
- WB: Wild-type HAP1, A431, C2C12, 2.4G2 and HeLa (ab150035) whole cell lysates, and rat brain tissue lysates. IHC-P: Human kidney carcinoma and breast carcinoma tissues.
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常规说明
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1720Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53277于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 63 kDa).
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| IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/50 |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 63 kDa). |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/50 |
靶标
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功能
Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle. -
组织特异性
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme. - Information by UniProt
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数据库链接
- Entrez Gene: 5597 Human
- Entrez Gene: 50772 Mouse
- Entrez Gene: 58840 Rat
- Omim: 602904 Human
- SwissProt: Q16659 Human
- SwissProt: Q61532 Mouse
- SwissProt: P27704 Rat
- Unigene: 411847 Human
see all -
别名
- ERK-3 antibody
- ERK3 antibody
- Extracellular signal regulated kinase 3 antibody
see all
图片
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Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)All lanes : Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MAPK6 knockout HeLa cell lysate
Lane 3 : 2.4G2 (Rat B cell lymphoma B lymphocyte) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab53277 observed at 90 kDa. Red - loading control ab8245 observed at 36 kDa.
ab53277 Anti-MAPK6/ERK3 antibody [EP1720Y] was shown to specifically react with MAPK6/ERK3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264910 (knockout cell lysate ab257526) was used. Wild-type and MAPK6/ERK3 knockout samples were subjected to SDS-PAGE. ab53277 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.cn/ps/products/53/ab53277/Images/ab53277-5-anti-mapk6-erk-3-antibody-ep1720y-immunohistochemistry-kidney-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling MAPK6/ERK3 with Purified ab53277 at 1:100 dilution (13.52 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.cn/ps/products/53/ab53277/Images/ab53277-369828-anti-mapk6erk-3-antibody-ep1720y-western-blot-hap1-wt-hap1-mapk6-knockout-hap1-ccne2-knockout-a431.jpg)
Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)All lanes : Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MAPK6 knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab53277 observed at 90 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab53277 was shown to react with MAPK6 in HAP1 wild-type cells in Western blot. Loss of signal was observed when MAPK6 knockout sample was used. HAP1 wild-type and MAPK6 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab53277 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.cn/ps/products/53/ab53277/Images/ab53277-367562-anti-mapk6-erk-3-antibody-ep1720y-westernblot-human-mouse-rat.jpg)
Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)All lanes : Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Rat brain lysates
Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in PMID:30642948 and 30166347
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.cn/ps/products/53/ab53277/Images/ab53277-66048-anti-mapk6-erk-3-antibody-ep1720y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)Unpurified ab53277 (1:50) staining human MAPK6/ERK3 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.cn/ps/products/53/ab53277/Images/ab53277-8-benefits-of-recombinant-antibodies.png)
Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab53277 被引用在 25 文献中.
- Vallabhaneni S et al. Conditional ERK3 overexpression cooperates with PTEN deletion to promote lung adenocarcinoma formation in mice. Mol Oncol 16:1184-1199 (2022). PubMed: 34719109
- Huang Y et al. Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation. Int J Biol Sci 18:112-123 (2022). PubMed: 34975321
- Soulez M et al. ERK3-MK5 signaling regulates myogenic differentiation and muscle regeneration by promoting FoxO3 degradation. J Cell Physiol 237:2271-2287 (2022). PubMed: 35141958
- Brandolini L et al. Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions. Cell Death Dis 13:500 (2022). PubMed: 35614037
- Alshammari ES et al. ERK3 is transcriptionally upregulated by ?Np63a and mediates the role of ?Np63a in suppressing cell migration in non-melanoma skin cancers. BMC Cancer 21:155 (2021). PubMed: 33579235