Anti-MAP3K8/COT (phospho T290)抗体(ab51214)
Key features and details
- Rabbit polyclonal to MAP3K8/COT (phospho T290)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MAP3K8/COT (phospho T290)抗体
参阅全部 MAP3K8/COT 一抗 -
描述
兔多克隆抗体to MAP3K8/COT (phospho T290) -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MAP3K8/COT aa 250-350 (phospho T290).
Database link: P41279 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51214于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. -
组织特异性
Expressed in several normal tissues and human tumor-derived cell lines. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
发展阶段
Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle. -
翻译后修饰
Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1326 Human
- Omim: 191195 Human
- SwissProt: P41279 Human
- Unigene: 432453 Human
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别名
- AURA2 antibody
- c COT antibody
- Cancer Osaka thyroid oncogene antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP3K8/COT (phospho T290) antibody (ab51214)
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8/COT (phospho-Thr290) at a 1/50 dilution.
Left image: Un-treated
Right image: Treated with phosphopeptide
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab51214 被引用在 1 文献中.
- Jeong JH et al. TPL2/COT/MAP3K8 (TPL2) Activation Promotes Androgen Depletion-Independent (ADI) Prostate Cancer Growth. PLoS One 6:e16205 (2011). WB ; Human . PubMed: 21267413