JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308011

重组Anti-MAP3K8/COT抗体[RP23040075]

Anti-MAP3K8/COT antibody [RP23040075]

Be the first to review this product! Submit a review

|

(0 Publication)

Anti-MAP3K8/COT antibody [RP23040075] is a Rabbit Recombinant Multiclonal antibody that is used in MAP3K8/COT ICC/IF, Western Blot. Suitable for Human, Mouse samples.

MAP3K8 is protein kinase which activated by phosphorylation during the S and G2/M phases of the cell cycle. MAP3K8 has been identified as an oncogene and can activate both MAP kinase and JNLK kinase pathways.

查看别名

COT, ESTF, MAP3K8, Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)

Immunofluorescence analysis of 4% paraformaldehyde on 70% confluent log phase U-87MG cells  labelling COT/MAP3K8 with ab308011 at 2 µg/mL, followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1 : 300 dilution. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Western blot - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)
  • WB

Supplier Data

Western blot - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)

Western blot analysis of MAP3K8/COT in whole cell extracts from Jurkat cells (lane 1), 3T3L1 cells (lane 2), and HepG2 cells (lane 3) using ab308011 at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~52kDa.

All lanes:

Western blot - Anti-MAP3K8/COT antibody [RP23040075] (ab308011) at 2 µg/mL

Lane 1:

Jurkat cells whole cell lysate

Lane 2:

3T3L1 whole cell lysate

Lane 3:

HepG2 whole cell lysate

Observed band size: 52 kDa

true

关键信息

宿主种属

Rabbit

克隆

Multiclonal

克隆号

RP23040075

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse

应用

ICC/IF, WB

applications

免疫原

Synthetic Peptide within Human MAP3K8.

P41279

style
left-column

搭配使用产品

Primary Antibodies

AB186733

Anti-Argonaute-2 antibody [EPR10411]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)

  • 0
  • 0
Primary Antibodies

AB192589

Anti-FGFR4 (phospho Y642) antibody

Western blot - Anti-FGFR4 (phospho Y642) antibody (AB192589)

  • 0
  • 0
Primary Antibodies

AB203568

Anti-NFkB Inducing Kinase NIK antibody [3H31L48]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (AB203568)

  • 0
  • 0
Primary Antibodies

AB193081

Anti-MYSM1 antibody [EPR18657]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (AB193081)

  • 0
  • 1
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

产品详情

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

style
left-column

性能和储存信息

形式
Liquid
存储溶液
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.
See full target information MAP3K8
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com