重组Anti-MAP3K4抗体[EP551Y] (ab40784)
![Western blot - Anti-MAP3K4 antibody [EP551Y] (ab40784)](https://www.abcam.cn/ps/products/40/ab40784/Images/ab40784-53956-anti-map3k4-antibody-ep551y-western-blot.jpg "Western blot - Anti-MAP3K4 antibody [EP551Y] (ab40784)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP551Y] to MAP3K4
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MAP3K4抗体[EP551Y]
参阅全部 MAP3K4 一抗 -
描述
兔单克隆抗体[EP551Y] to MAP3K4 -
宿主
Rabbit -
特异性
This antibody is predicted to detect splice isoform 2 based on sequence analysis. -
经测试应用
适用于: IHC-P, WBmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MAP3K4 aa 150-250.
Database link: Q9Y6R4 -
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP551Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40784于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 182 kDa).
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 182 kDa). |
靶标
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功能
Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6. -
组织特异性
Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm > perinuclear region. Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 4216 Human
- Omim: 602425 Human
- SwissProt: Q9Y6R4 Human
- Unigene: 390428 Human
-
别名
- dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4) antibody
- FLJ42439 antibody
- KIAA0213 antibody
see all
图片
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Western blot - Anti-MAP3K4 antibody [EP551Y] (ab40784)Anti-MAP3K4 antibody [EP551Y] (ab40784) at 1/1000 dilution + 293 cell lysate. at 10 µg
Predicted band size: 182 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP3K4 antibody [EP551Y] (ab40784)](https://www.abcam.cn/ps/products/40/ab40784/Images/ab40784-53955-anti-map3k4-antibody-ep551y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP3K4 antibody [EP551Y] (ab40784)Immunohistochemical analysis of paraffin-embedded human ovary adenocarcinoma using ab40784.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-MAP3K4 antibody [EP551Y] (ab40784)](https://www.abcam.cn/ps/products/40/ab40784/Images/ab40784-3-benefits-of-recombinant-antibodies.png)
Anti-MAP3K4 antibody [EP551Y] (ab40784)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab40784 被引用在 1 文献中.
- Duan JL et al. A novel peptide encoded by N6-methyladenosine modified circMAP3K4 prevents apoptosis in hepatocellular carcinoma. Mol Cancer 21:93 (2022). PubMed: 35366894