重组Anti-MAP3K4抗体[EP551Y] (ab40784)
![Western blot - Anti-MAP3K4 antibody [EP551Y] (ab40784)](https://www.abcam.cn/ps/products/40/ab40784/Images/ab40784-53956-anti-map3k4-antibody-ep551y-western-blot.jpg "Western blot - Anti-MAP3K4 antibody [EP551Y] (ab40784)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP551Y] to MAP3K4
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MAP3K4抗体[EP551Y]
参阅全部 MAP3K4 一抗 -
描述
兔单克隆抗体[EP551Y] to MAP3K4 -
宿主
Rabbit -
特异性
This antibody is predicted to detect splice isoform 2 based on sequence analysis. -
经测试应用
适用于: IHC-P, WBmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MAP3K4 aa 150-250.
Database link: Q9Y6R4 -
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP551Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40784于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 182 kDa).
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 182 kDa). |
靶标
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功能
Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6. -
组织特异性
Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm > perinuclear region. Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 4216 Human
- Omim: 602425 Human
- SwissProt: Q9Y6R4 Human
- Unigene: 390428 Human
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别名
- dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4) antibody
- FLJ42439 antibody
- KIAA0213 antibody
see all
图片
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Western blot - Anti-MAP3K4 antibody [EP551Y] (ab40784)Anti-MAP3K4 antibody [EP551Y] (ab40784) at 1/1000 dilution + 293 cell lysate. at 10 µg
Predicted band size: 182 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP3K4 antibody [EP551Y] (ab40784)](https://www.abcam.cn/ps/products/40/ab40784/Images/ab40784-53955-anti-map3k4-antibody-ep551y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP3K4 antibody [EP551Y] (ab40784)Immunohistochemical analysis of paraffin-embedded human ovary adenocarcinoma using ab40784.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-MAP3K4 antibody [EP551Y] (ab40784)](https://www.abcam.cn/ps/products/40/ab40784/Images/ab40784-3-benefits-of-recombinant-antibodies.png)
Anti-MAP3K4 antibody [EP551Y] (ab40784)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab40784 被引用在 1 文献中.
- Duan JL et al. A novel peptide encoded by N6-methyladenosine modified circMAP3K4 prevents apoptosis in hepatocellular carcinoma. Mol Cancer 21:93 (2022). PubMed: 35366894