重组Anti-MAP2 (phospho S136)抗体[EPR2361] (ab96378)
![Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)](https://www.abcam.cn/ps/products/96/ab96378/Images/ab96378-110750-anti-map2-phospho-s136-antibody-epr2361-western-blot.jpg "Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2361] to MAP2 (phospho S136)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-MAP2 (phospho S136)抗体[EPR2361]
参阅全部 MAP2 一抗 -
描述
兔单克隆抗体[EPR2361] to MAP2 (phospho S136) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal brain and mouse brain tissue lysates IHC-P: Human brain tissue ICC/IF: Mouse primary neuron + Alkaline Phosphatase cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2361 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96378于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 200 kDa.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF | (1) |
1/500.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 200 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/500. |
靶标
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功能
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
序列相似性
Contains 3 Tau/MAP repeats. -
翻译后修饰
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
细胞定位
Cytoplasm, cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 4133 Human
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 368281 Human
see all -
别名
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
图片
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Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)All lanes : Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378) at 1/1000 dilution
Lane 1 : Untreated fetal brain lysates
Lane 2 : Fetal brain lysates treated with Alkaline Phosphatase
Lane 3 : Mouse brain lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP/AP polymerized antibody
Predicted band size: 200 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)](https://www.abcam.cn/ps/products/96/ab96378/Images/ab96378-3-anti-map2-phospho-s136-antibody-epr2361-immunocytochemistry-neuron-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron + Alkaline Phosphatase cells labeling MAP2 with purified ab96378 at 1/500 (3 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)](https://www.abcam.cn/ps/products/96/ab96378/Images/ab96378-110749-anti-map2-phospho-s136-antibody-epr2361-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)ab96378, at a 1/100 dilution, staining MAP2 in paraffin embedded Human brain tissue by Immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)](https://www.abcam.cn/ps/products/96/ab96378/Images/ab96378-4-benefits-of-recombinant-antibodies.png)
Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab96378 被引用在 3 文献中.
- Wang W et al. Toxic amyloid-ß oligomers induced self-replication in astrocytes triggering neuronal injury. EBioMedicine 42:174-187 (2019). PubMed: 30926423
- Alvarez-Carbonell D et al. Cross-talk between microglia and neurons regulates HIV latency. PLoS Pathog 15:e1008249 (2019). PubMed: 31887215
- Ni N et al. Effects of let-7b and TLX on the proliferation and differentiation of retinal progenitor cells in vitro. Sci Rep 4:6671 (2014). ICC ; Mouse . PubMed: 25327364