为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Neuroscience Cell Type Marker Neuron marker Soma marker
重组RabMAb

重组Anti-MAP2 (phospho S136)抗体[EPR2361] (ab96378)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (4)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)
  • Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2361] to MAP2 (phospho S136)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

你可能会感兴趣

蛋白
Product image
Recombinant Human MAP2 protein (ab114686)
二抗
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
一抗
Product image
Anti-mu Crystallin antibody (ab220085)

查看更多相关产品

概述

  • 产品名称

    Anti-MAP2 (phospho S136)抗体[EPR2361]
    参阅全部 MAP2 一抗
  • 描述

    兔单克隆抗体[EPR2361] to MAP2 (phospho S136)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human fetal brain and mouse brain tissue lysates IHC-P: Human brain tissue ICC/IF: Mouse primary neuron + Alkaline Phosphatase cells
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR2361
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage

相关产品

  • Alternative Versions

    • Anti-MAP2 (phospho S136) antibody [EPR2361] - BSA and Azide free (ab247601)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human MAP2 protein (ab114686)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab96378于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 200 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
1/500.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 200 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/500.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • 序列相似性

    Contains 3 Tau/MAP repeats.
  • 翻译后修饰

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • 细胞定位

    Cytoplasm, cytoskeleton.
  • Target information above from: UniProt accession P11137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4133 Human
    • Entrez Gene: 17756 Mouse
    • Entrez Gene: 25595 Rat
    • Omim: 157130 Human
    • SwissProt: P11137 Human
    • SwissProt: P20357 Mouse
    • SwissProt: P15146 Rat
    • Unigene: 368281 Human
    • Unigene: 256966 Mouse
    • Unigene: 436793 Mouse
    • Unigene: 10484 Rat
    • Unigene: 136301 Rat
    • Unigene: 230443 Rat
    see all
  • 别名

    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

图片

  • Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)
    Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)
    All lanes : Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378) at 1/1000 dilution

    Lane 1 : Untreated fetal brain lysates
    Lane 2 : Fetal brain lysates treated with Alkaline Phosphatase
    Lane 3 : Mouse brain lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP/AP polymerized antibody

    Predicted band size: 200 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron + Alkaline Phosphatase cells labeling MAP2 with purified ab96378 at 1/500 (3 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

    ab96378, at a 1/100 dilution, staining MAP2 in paraffin embedded Human brain tissue by Immunohistochemical analysis.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)
    Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (4)

发表研究结果有使用 ab96378?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab96378 被引用在 4 文献中.

  • Gabriel-Segard T  et al. Proof-of-Concept Human Organ-on-Chip Study: First Step of Platform to Assess Neuro-Immunological Communication Involved in Inflammatory Bowel Diseases. Int J Mol Sci 24:N/A (2023). PubMed: 37445748
  • Wang W  et al. Toxic amyloid-ß oligomers induced self-replication in astrocytes triggering neuronal injury. EBioMedicine 42:174-187 (2019). PubMed: 30926423
  • Alvarez-Carbonell D  et al. Cross-talk between microglia and neurons regulates HIV latency. PLoS Pathog 15:e1008249 (2019). PubMed: 31887215
  • Ni N  et al. Effects of let-7b and TLX on the proliferation and differentiation of retinal progenitor cells in vitro. Sci Rep 4:6671 (2014). ICC ; Mouse . PubMed: 25327364

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 (phospho S136) antibody [EPR2361]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
Specification
Cryopreserved embryonic cortical neurons (QBMcells
Permeabilization
No
Fixative
paraformaldehyde with picric acid
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Babben Tinner

Verified customer

提交于 Feb 19 2015

Immunohistochemistry (Frozen sections) abreview for Anti-MAP2 (phospho S136) antibody [EPR2361]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Inner plexiform layer)
Specification
Retina, Inner plexiform layer
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. XXXXXX XXXXXX

Verified customer

提交于 Nov 17 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2025 艾博抗(上海)贸易有限公司版权所有