重组Anti-MAP2抗体[RM1010] - Neuronal Marker

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Mouse primary neuron cells labelling MAP2 with 281588 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling MAP2 with 281588 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling MAP2 with 281588 at 1/100 (5.52 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling MAP2 with 281588 at 1/100 (5.52 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• ICC

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Immunocytochemistry - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with 281588 at 1/2000 (0.276 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• WB

Lab

Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588) at 1/1000 dilution

Lane 1:

SK-N-BE(2) (Human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 2:

IMR-32 (Human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 3:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 199 kDa

Observed band size: 280 kDa,70 kDa

false

• WB

Supplier Data

Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588) at 1/1000 dilution

Lane 1:

Mouse E12.5 brain lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Mouse cerebellum lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Lane 5:

Rat cerebellum lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 199 kDa

Observed band size: 280 kDa,70 kDa

false