Anti-MAP2抗体[AP-20] (ab11268)
Key features and details
- Mouse monoclonal [AP-20] to MAP2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse
- Isotype: IgG1
概述
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产品名称
Anti-MAP2抗体[AP-20]
参阅全部 MAP2 一抗 -
描述
小鼠单克隆抗体[AP-20] to MAP2 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Full length native protein (purified) corresponding to Cow MAP2.
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阳性对照
- ICC: Mouse Cell (Cortical neuron culture)
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常规说明
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR143561. Lot numbers higher than GR143561 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Purified from Tissue culture supernatant. -
克隆
单克隆 -
克隆编号
AP-20 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11268于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/5000.
Use at an assay dependent concentration. |
说明 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/5000. Use at an assay dependent concentration. |
靶标
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功能
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
序列相似性
Contains 3 Tau/MAP repeats. -
翻译后修饰
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
细胞定位
Cytoplasm, cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 17756 Mouse
- SwissProt: P20357 Mouse
- Unigene: 256966 Mouse
- Unigene: 436793 Mouse
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别名
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
图片
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Mouse monoclonal [AP-20] to MAP2 - Neuronal Marker (ab11268) used at a dilution of 1/500 in Immunocytochemistry/Immunofluorescence on Mouse Cell (Cortical neuron culture). Fixative: Paraformaldehyde. Blocking step: 2% Serum incubated for 1 hour at RT. Secondary Antibody: Goat anti-mouse AlexaFluor 594 Conjugation: Alexa Fluor® 594; 1/1000
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ab11268 staining MAP2 in E16 mouse brain neocortex (bottom is dorsal) by immunohistochemistry. Free-floating sections were incubated with ab11268 (1/5000) for 2 hours at 21ºC. Bound antibody was detected using a biotinylated donkey anti-mouse IgG antibody. This image is courtesy of an Abreview submitted by Randal Moldrich, 6 March 2008.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab11268 被引用在 36 文献中.
- Yang RY et al. Knockdown of polypyrimidine tract binding protein facilitates motor function recovery after spinal cord injury. Neural Regen Res 18:396-403 (2023). PubMed: 35900436
- Shikanai M et al. Rab21 regulates caveolin-1-mediated endocytic trafficking to promote immature neurite pruning. EMBO Rep 24:e54701 (2023). PubMed: 36683567
- Ali H et al. Attenuation hotspots in neurotropic human astroviruses. PLoS Biol 21:e3001815 (2023). PubMed: 37459343
- Solana-Balaguer J et al. Neuron-derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB-mediated signalling and preserve neuronal complexity. J Extracell Vesicles 12:e12355 (2023). PubMed: 37743539
- Solana-Balaguer J et al. RTP801 mediates transneuronal toxicity in culture via extracellular vesicles. J Extracell Vesicles 12:e12378 (2023). PubMed: 37932242